Molecular cloning of serine proteases from elapid snake venoms

	Molecular cloning of serine proteases from elapid snake venoms
	Jin Y; Lee WH; Zhang Y*; zhangy@mail.kiz.ac.cn
	2007
发表期刊	TOXICON
ISSN	0041-0101
卷号	49 期号:8 页码:1200-1207
合作性质	其它
摘要	Serine proteases are widely distributed in viperid snake venoms, but rare in elapid snake venoms. Previously, we have identified a fibrinogenolytic enzyme termed OhS1 from the venom of Ophiophagus hannah. The results indicated that OhS1 might be a serine protease, but there was no structural evidence previously. In the present study, the primary structure of OhS1 was determined by protein sequencing, in combination with RT-PCR and 5'-RACE methods. OhS1 precursor is composed of an 18-amino acid signal peptide, a 6-amino acid putative activation peptide and 236-amino acid mature protein. OhS1 homologues from Naja atra and Bungarus multicinctus were also cloned and reported. These elapid venom serine proteases exhibited approximately 60% sequence identity with serine proteases from the snake venoms of the Viperidae and Colubridae family. Phylogenetic analysis indicated that snake venom serine protease might have a common ancestor.
关键词	Serine Protease Elapidae Cloning
资助者	This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23)
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23) ; This work was supported by grants from Na- tional Natural Science Foundation (30630014, 30570359, 30470380 and 30670412) and the grants of ‘‘Western Light’’ Projects. Dr. Lee also received a grant from Yunnan Science and Technology Com- mission (2005PY01-23)
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2699
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	Totoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Jin Y,Lee WH,Zhang Y*,et al. Molecular cloning of serine proteases from elapid snake venoms[J]. TOXICON,2007,49(8):1200-1207.
APA	Jin Y,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2007).Molecular cloning of serine proteases from elapid snake venoms.TOXICON,49(8),1200-1207.
MLA	Jin Y,et al."Molecular cloning of serine proteases from elapid snake venoms".TOXICON 49.8(2007):1200-1207.

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