Isolation and cloning of a metalloproteinase from king cobra snake venom

	Isolation and cloning of a metalloproteinase from king cobra snake venom
	Guo XX 1,2; Zeng L 1,2; Lee WH 1; Zhang Y*1; Jin Y 1; zhangy@mail.kiz.ac.cn
	2007
发表期刊	TOXICON
ISSN	0041-0101
卷号	49 期号:7 页码:954-965
合作性质	其它
摘要	A 50 kDa fibrinogenolytic protease, ohagin, from the venom of Ophiophagus hannah was isolated by a combination of gel filtration, ion-exchange and heparin affinity chromatography. Ohagin specifically degraded the alpha-chain of human fibrinogen and the proteolytic activity was completely abolished by EDTA, but not by PMSF, suggesting it is a metalloproteinase. It dose-dependently inhibited platelet aggregation induced by ADP, TMVA and stejnulxin. The full sequence of ohagin was deduced by cDNA cloning and confirmed by protein sequencing and peptide mass fingerprinting. The full-length cDNA sequence of ohagin encodes an open reading frame of 611 amino acids that includes signal peptide, proprotein and mature protein comprising metalloproteinase, disintegrin-like and cysteine-rich domains, suggesting it belongs to P-III class metalloproteinase. In addition, P-III class metalloproteinases from the venom glands of Naja atra, Bungarus multicinctus and Bungarus fasciatus were also cloned in this study. Sequence analysis and phylogenetic analysis indicated that metalloproteinases from elapid snake venoms form a new subgroup of P-III SVMPs.
关键词	Metalloproteinase Elapid Ohagin Snake Venom
资助者	This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects
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收录类别	SCI
语种	英语
资助者	This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects ; This work was supported by Grants from National Natural Science Foundation of China (30630014, 30670412, 30570359 and 30470380), Yunnan Natural Science Foundation (2006C0047Q) and the Grants of ‘‘Western Light’’ Projects
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2701
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Guo XX,Zeng L,Lee WH,et al. Isolation and cloning of a metalloproteinase from king cobra snake venom[J]. TOXICON,2007,49(7):954-965.
APA	Guo XX,Zeng L,Lee WH,Zhang Y*,Jin Y,&zhangy@mail.kiz.ac.cn.(2007).Isolation and cloning of a metalloproteinase from king cobra snake venom.TOXICON,49(7),954-965.
MLA	Guo XX,et al."Isolation and cloning of a metalloproteinase from king cobra snake venom".TOXICON 49.7(2007):954-965.

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