Identification and characterization of novel reptile cathelicidins from elapid snakes

	Identification and characterization of novel reptile cathelicidins from elapid snakes
	Zhao H 1,2; Gan TX 1,3; Liu XD 2; Jin Y 1; Lee WH 1; Shen JH2; Zhang Y1; kmsjh99@yahoo.com.cn ; zhangy@mail.kiz.ac.cn
	2008
发表期刊	PEPTIDES
ISSN	0196-9781
卷号	29 期号:10 页码:1685-1691
合作性质	其它
摘要	Three cDNA sequences coding for elapid cathelicidins were cloned from constructed venom gland cDNA libraries of Naja atra, Bungarus fasciatus and Ophiophagus hannah. The open reading frames of the cloned elapid cathelicidins were all composed of 576 bp and coded for 191 amino acid residue protein precursors. Each of the deduced elapid cathelicidin has a 22 amino acid residue signal peptide, a conserved cathelin domain of 135 amino acid residues and a mature antimicrobial peptide of 34 amino acid residues. Unlike the highly divergent cathelicidins in mammals, the nucleotide and deduced protein sequences of the three cloned elapid cathelicidins were remarkably conserved. All the elapid mature cathelicidins were predicted to be cleaved at Valine157 by elastase. OH-CATH, the deduced mature cathelicidin from king cobra, was chemically synthesized and it showed strong antibacterial activity against various bacteria with minimal inhibitory concentration of 1-20 mu g/ml in the presence of 1% NaCl. Meanwhile, the synthetic peptide showed no haemolytic activity toward human red blood cells even at a high dose of 200 mu g/ml. Phylogenetic analysis of cathelicidins from vertebrate suggested that elapid and viperid cathelicidins were grouped together in the tree. Snake cathelicidins were evolutionary closely related to the neutrophilic granule proteins (NGPs) from mouse, rat and rabbit. Snake cathelicidins also showed a close relationship with avian fowlicidins (1-3) and chicken myeloid antimicrobial peptide 27. Elapid cathelicidins might be used as models for the development of novel therapeutic drugs.
关键词	Antimicrobial Peptide Cathelicidin Elapid Snake Molecular Cloning Phylogenetic Analysis
资助者	This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R).
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R). ; This work was supported by the grants of ‘‘Key Research Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects from The Chinese Academy of Sciences; and grants from National Natural Science Founda- tion(30670412,30630014)andYunnanScienceandTechnology Commission (2006PT08-1, 2006C0047Q, 2007C0006R).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2707
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	kmsjh99@yahoo.com.cn; zhangy@mail.kiz.ac.cn
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Department of Urology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China 3.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Zhao H,Gan TX,Liu XD,et al. Identification and characterization of novel reptile cathelicidins from elapid snakes[J]. PEPTIDES,2008,29(10):1685-1691.
APA	Zhao H.,Gan TX.,Liu XD.,Jin Y.,Lee WH.,...&zhangy@mail.kiz.ac.cn.(2008).Identification and characterization of novel reptile cathelicidins from elapid snakes.PEPTIDES,29(10),1685-1691.
MLA	Zhao H,et al."Identification and characterization of novel reptile cathelicidins from elapid snakes".PEPTIDES 29.10(2008):1685-1691.

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