Melanoma cell growth inhibition by beta gamma-CAT, which is a novel non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin

	Melanoma cell growth inhibition by beta gamma-CAT, which is a novel non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin
	He YY 1,2; Liu SB 1,2; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2008
发表期刊	TOXICON
ISSN	0041-0101
卷号	52 期号:2 页码:341-347
合作性质	其它
摘要	In vertebrates, non-lens beta gamma-crystallins are widely expressed in various tissues but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive. beta gamma-CAT, which is responsible for the potent hemolytic activity and lethal toxicity on mice of frog Bombina maxima skin secretions, is the first example of a non-lens beta gamma-crystallin and trefoil factor complex. Its alpha- and beta-subunits show significant sequence homology to human Absent In Melanoma I and trefoil factors, respectively. Here, we showed that fly-CAT triggered two types of cellular responses in human melanoma cells. On one hand, the protein (25-200 pM) was able to stimulate cell migration in melanoma A375 cells. On the other hand, it inhibited cell proliferation by delaying S-G2/M cell phase transition. Blockade of the cell cycle was associated with increased p21/WAFI expression, and reduced amounts of Cdc2 and Cdc25C. Py-CAT also reduced Cdc2 function by increasing the level of inactivated phospho-Cdc2. In addition, the expression of JunB, a well-known cell proliferation inhibitor, was significantly up-regulated. These results provide the first evidence of an anti-proliferative role for a non-lens beta gamma-crystallin member and action mechanism via association with a trefoil factor.
关键词	Non-lens Beta Gamma-crystallin Trefoil Factor Cell Migration Cell Proliferation Cell Cycle Melanoma Beta Gamma-cat
资助者	This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang.
收录类别	SCI
语种	英语
资助者	This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang. ; This work was supported by grants from The Chinese National Natural Science Foundation (30630014 and 30570359), and a grant of ‘‘Key Research Direction’’ (KSCX2-YW-R-088) from The Chinese Academy of Sciences to Y. Zhang.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2713
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	He YY,Liu SB,Lee WH,et al. Melanoma cell growth inhibition by beta gamma-CAT, which is a novel non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin[J]. TOXICON,2008,52(2):341-347.
APA	He YY,Liu SB,Lee WH,Zhang Y*,&zhangy@mail.kiz.ac.cn.(2008).Melanoma cell growth inhibition by beta gamma-CAT, which is a novel non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin.TOXICON,52(2),341-347.
MLA	He YY,et al."Melanoma cell growth inhibition by beta gamma-CAT, which is a novel non-lens betagamma-crystallin and trefoil factor complex from frog Bombina maxima skin".TOXICON 52.2(2008):341-347.

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