Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake

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	Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake
	Lu QM; Jin Y; Wei JF; Li DS; Zhu SW; Wang WY; Xiong YL*; weiqinwq@yahoo.com.cn, wangwy@mail. kiz.ac.cn
	2002
发表期刊	TOXICON
ISSN	0041-0101
卷号	40 期号:9 页码:1313-1319
合作性质	其它
摘要	A phospholipase A(2) (PLA(2)), called jerdoxin, was isolated from Trimeresurus jerdonni snake venom and partially characterized. The protein was purified by three chromatographic steps. SDS-polyacrylamide gel electrophoresis in the presence or absence of dithiothreitol showed that it had a molecular mass of 15 kDa. Jerdoxin had an enzymatic activity of 39.4 micro mol/min/mg towards egg yolk phosphatidyl choline (PC). It induced edema in the footpads of mice. In addition, jerdoxin exhibited indirect hemolytic activity. About 97% hemolysis was observed when 2 micro g/ml enzyme was incubated for 90 min in the presence of PC and Ca(2+). No detectable hemolysis was noticed when PC was not added. Ca(2+) was necessary for jerdoxin to exert its hemolytic activity, since only 52% hemolysis was seen when Ca(2+) was absent in the reaction mixture. Furthermore, jerdoxin inhibited ADP induced rabbit platelet aggregation and the inhibition was dose dependent with an IC(50) of 1.0 micro M. The complete amino acid sequence of jerdoxin deduced from cDNA sequence shared high homology with other snake venom PLA(2)s, especially the D 49 PLA(2)s. Also, the residues concerned to Ca(2+) binding were conserved. This is the first report of cDNA sequence of T. jerdonii venom PLA(2).
关键词	Trimeresurus Jerdonii Jerdoxin Phosphatidyl Choline
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2793
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	weiqinwq@yahoo.com.cn, wangwy@mail. kiz.ac.cn
作者单位	Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China
推荐引用方式 GB/T 7714	Lu QM,Jin Y,Wei JF,et al. Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake[J]. TOXICON,2002,40(9):1313-1319.
APA	Lu QM.,Jin Y.,Wei JF.,Li DS.,Zhu SW.,...&weiqinwq@yahoo.com.cn, wangwy@mail. kiz.ac.cn.(2002).Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake.TOXICON,40(9),1313-1319.
MLA	Lu QM,et al."Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake".TOXICON 40.9(2002):1313-1319.

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