Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom

KIZ OpenIR > 其他

	Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom
	Wei Q 1,2; Lu QM 1; Jin Y 1,2; Li R 1; Wei JF 1,2; Wang WY 1; Xiong YL*1; weiqinwq@yahoo.com.cn
	2002
发表期刊	TOXICON
ISSN	0041-0101
卷号	40 期号:9 页码:1331-1338
合作性质	其它
摘要	TMVA, a novel C-type lectin-like protein that induces platelet aggregation in a dose-dependent manner, was purified from the venom of Trimeresurus mucrosquamatus. It consists of two subunits, alpha (15,536 Da) and beta (14,873 Da). The mature amino acid sequences of the a (135 amino acids) and beta subunits (123 amino acids) were deduced from cloned cDNAs. Both of the sequences show great similarity to C-type lectin-like venom proteins, including a carbohydrate recognition domain. The cysteine residues of TMVA are conserved at positions corresponding to those of flavocetin-A and convulxin, including the additional Cys135 in the alpha subunit and Cys3 in the beta subunit. SDS-PAGE, mass spectrometry analysis and amino acid sequence showed that native TMVA exists as two convertible multimers Of (alphabeta)(2) and (alphabeta)(4) with molecular weights of 63,680 and 128,518 Da, respectively. The (alphabeta)(2) complex is stabilized by an interchain disulfide bridge between the two alphabeta-heterodimers, whereas the stabilization of the (alphabeta)(4) complex seems to involve non-covalent interactions between the (alphabeta)(2) complexes.
关键词	C-type Lectin-like Protein Platelet Aggregation Heterodimer Trimeresurus Mucrosquamatus
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2795
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	weiqinwq@yahoo.com.cn
作者单位	1.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China 2.Graduate School of the Chinese Academy of Science, People’s Republic of China
推荐引用方式 GB/T 7714	Wei Q,Lu QM,Jin Y,et al. Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom[J]. TOXICON,2002,40(9):1331-1338.
APA	Wei Q.,Lu QM.,Jin Y.,Li R.,Wei JF.,...&weiqinwq@yahoo.com.cn.(2002).Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom.TOXICON,40(9),1331-1338.
MLA	Wei Q,et al."Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom".TOXICON 40.9(2002):1331-1338.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2002401331.pdf（1084KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文