Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen

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	Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen
	Jin Y 1,2; Lu QM 1; Wang WY 1; Xiong YL*1; luqiumin@hotmail.com
	2002
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B
ISSN	1096-4959
卷号	132 期号:3 页码:529-534
合作性质	其它
摘要	Jerdonobin and jerdofibrase are two serine proteases purified from the venom of Trimeresurus jerdonii. The Michaelis constant K-m and the catalytic rate constant K-cat of jerdonobin or jerdofibrase on three chromogenic substrates, H-D-Pro-Phe-Arg-pNA (S2302), H-D-Phe-pipecolyl-Arg-pNA (S2238), and H-D-Val-Leu-Lys-pNA (S2251) were obtained from lineweaver-Burk plots. Jerdofibrase could hydrolyze all three substrates, but jerdonobin had no detectable activity on S2251, suggesting a relatively broader substrate specificity for jerdofibrase than jerdonobin. By SDS-PAGE, jerdofibrase preferentially degraded Bbeta-chain of fibrinogen. It also degraded Aalpha-chain of fibrinogen with relatively slow activity, but did not act on the gamma-chain. In contrast, jerdonobin did not degrade fibrinogen within 12 h. Fibrinopeptides liberation test, identified by HPLC, showed jerdonobin released fibrinopeptide A and a small amount of fibrinopeptide B. Unlike jerdonobin, jerdofibrase mainly released fibrinopeptide B. These results indicate that the two enzymes differ in their ability to hydrolyze chromogenic substrates and in their actions on fibrinogen.
关键词	Chromogenic Substrates Fibrinogen Fibrinopeptide Snake Venom Serine Protease Thrombin-like Enzyme Trimeresurus Jerdonii
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收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2799
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	luqiumin@hotmail.com
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, Kunming 650223, Yunnan, PR China a 2.Graduate School of Chinese Academy of Sciences, Yunnan, PR China
推荐引用方式 GB/T 7714	Jin Y,Lu QM,Wang WY,et al. Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,2002,132(3):529-534.
APA	Jin Y,Lu QM,Wang WY,Xiong YL*,&luqiumin@hotmail.com.(2002).Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,132(3),529-534.
MLA	Jin Y,et al."Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B 132.3(2002):529-534.

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