A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling

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	A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling
	Meng QX; Wang WY; Lu QM; Jin Y; Wei JF; Zhu SW; Xiong YL*; qx_meng@hotmail.com
	2002
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN	1532-0456
卷号	132 期号:1 页码:113-121
合作性质	其它
摘要	A novel short neurotoxin, cobrotoxin c (CBT C) was isolated from the venom of monocellate cobra (Naja kaouthia) using a combination of ion-exchange chromatography and FPLC. Its primary structure was determined by Edman degradation. CBT C is composed of 61 amino acid residues. It differs from cobrotoxin b (CBT B) by only two amino acid substitutions, Thr/Ala11 and Arg/Thr56, which are not located on the functionally important regions by sequence similarity. However, the LD50 is 0.08 mg/g to mice, i.e. approximately five-fold higher than for CBT B. Strikingly, a structure-function relationship analysis suggests the existence of a functionally important domain on the outside of Loop III of CBT C. The functionally important basic residues on the outside of Loop III might have a pairwise interaction with alpha subunit, instead of gamma or delta subunits of the nicotinic acetylcholine receptor (nAChR).
关键词	Snake Venom Short Neurotoxin Naja Kaouthia Cobrotoxin c Amino Acid Sequence Three-finger Proteins Lethal Toxicity Comparative Structure Functional Domain
资助者	The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165).
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收录类别	SCI
语种	英语
资助者	The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165). ; The project was supported by the National Nature Science Fund of China (Project number: 39670165).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2801
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	qx_meng@hotmail.com
作者单位	Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China
推荐引用方式 GB/T 7714	Meng QX,Wang WY,Lu QM,et al. A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2002,132(1):113-121.
APA	Meng QX.,Wang WY.,Lu QM.,Jin Y.,Wei JF.,...&qx_meng@hotmail.com.(2002).A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,132(1),113-121.
MLA	Meng QX,et al."A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 132.1(2002):113-121.

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