A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding

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	A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding
	Zhou XD 1,2,3; Ding CH 1,2; Tai H 1; Jin Y 1; Chen RQ 1,2; Lu QM 1; Wang WY 1; Xiong YL*1; toxinlab@mail.kiz.ac.cn
	2004
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	139 期号:1 页码:117-122
摘要	A novel disintegrin, jerdonatin, was purified to homogeneity from Trimeresurus jerdonii venom by gel filtration and reversed-phase high-pressure liquid chromatography. We isolated the cDNA encoding jerdonatin from the snake venom gland. Jerdonatin cDNA precursor encoded pre-peptide, metalloprotease and disintegrin domain. Jerdonatin is composed of 72 amino acid residues including 12 cysteines and the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Molecular mass of jerdonatin was determined to be 8011 Da by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Jerdonatin inhibited ADP- and collagen-induced human platelet aggregation with IC50 of 123 and 135 nM, respectively. We also investigated the effect of jerdonatin on the binding of B6D2F1 hybrid mice spermatozoa to mice zona-free eggs and their subsequent fusion. Jerdonatin significantly inhibited sperm-egg binding in a concentration-dependent manner, but had no effect on the fusion of sperm-egg. These results indicate that integrins on the egg play a role in mammalian fertilization.
关键词	Venom Trimeresurus Jerdonii Cdna Sequence Peptide Disintegrin Platelet Sperm–egg Binding Fertilization
资助者	This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M.
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收录类别	SCI
语种	英语
资助者	This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2805
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	toxinlab@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, No. 32 Jiaochangdonglu, Kunming 650223, Yunnan, PR China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100080, PR China 3.Faculty of Biological and Chemical Engineering, Kumming University of Science and Technology, Kunming 650224, Yunnan, PR China
推荐引用方式 GB/T 7714	Zhou XD,Ding CH,Tai H,et al. A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2004,139(1):117-122.
APA	Zhou XD.,Ding CH.,Tai H.,Jin Y.,Chen RQ.,...&toxinlab@mail.kiz.ac.cn.(2004).A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,139(1),117-122.
MLA	Zhou XD,et al."A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 139.1(2004):117-122.

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