| GPIb is involved in platelet aggregation induced by mucetin, a snake C-type lectin protein from Chinese habu (Trimeresurus mucrosquamatus) venom | |
| Lu QM1,2; Navdaev A1; Clemetson JM1; Clemetson KJ*1; clemetson@tki.unibe.ch | |
| 2004 | |
| 发表期刊 | THROMBOSIS AND HAEMOSTASIS
 |
| ISSN | 0340-6245 |
| 卷号 | 91期号:6页码:1168-1176 |
| 合作性质 | 其它 |
| 摘要 | Mucetin (Trimeresurus mucrosquamatus venom activator, TMVA) is a potent platelet activator purified from Chinese habu (Trimeresurus mucrosquamatus) venom. It belongs to the snake venom heterodimeric C-type lectin family and exists in several multimeric forms. We now show that binding to platelet glycoprotein (GP) lb is involved in mucetin-induced platelet aggregation. Antibodies against GPIb as well as the GPIb-blocking C-type lectin echicetin inhibited mucetin-induced platelet aggregation. Binding of GPIb was confirmed by affinity chromatography and Western blotting. Antibodies against GPVI inhibited convulxin- but not mucetin-induced aggregation. Signalling by mucetin involved rapid tyrosine phosphorylation of a number of proteins including Syk, Src, LAT and PLCgamma2. Mucetininduced phosphorylation of the Fcgamma chain of platelet was greatly promoted by inhibition of alpha(llb)beta(3) by the peptidomimetic EMD 132338, suggesting that phosphatases downstream Of alpha(llb)beta(3) activation are involved in dephosphorylation of Fcgamma. Unlike other multimeric snake C-type lectins that act via GPIb and only agglutinate platelets, mucetin activates alpha(llb)beta(3). Inhibition Of alpha(llb)beta(3) strongly reduced the aggregation response to mucetin, indicating that activation Of alpha(llb)beta(3) and binding of fibrinogen are involved in mucetin-induced platelet aggregation. Apyrase and aspirin also inhibit platelet aggregation induced by mucetin, suggesting that ADP and thromboxaneA(2) are involved in autocrine feedback. Sequence and structural comparison with closely related members of this protein family point to features that may be responsible for the functional differences. |
| 关键词 | Snake Venom C-type Lectin Platelets Gpib Tyrosine Phosphorylation |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/2809 |
| 专题 | 科研部门_天然药物功能蛋白质学科组（赖仞） |
| 通讯作者 | clemetson@tki.unibe.ch |
| 作者单位 | 1.Theodor Kocher Institute, University of Bern, Bern, Switzerland 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming,Yunnan, China |
| 推荐引用方式 GB/T 7714 | Lu QM,Navdaev A,Clemetson JM,et al. GPIb is involved in platelet aggregation induced by mucetin, a snake C-type lectin protein from Chinese habu (Trimeresurus mucrosquamatus) venom[J]. THROMBOSIS AND HAEMOSTASIS,2004,91(6):1168-1176. |
| APA | Lu QM,Navdaev A,Clemetson JM,Clemetson KJ*,&clemetson@tki.unibe.ch.(2004).GPIb is involved in platelet aggregation induced by mucetin, a snake C-type lectin protein from Chinese habu (Trimeresurus mucrosquamatus) venom.THROMBOSIS AND HAEMOSTASIS,91(6),1168-1176. |
| MLA | Lu QM,et al."GPIb is involved in platelet aggregation induced by mucetin, a snake C-type lectin protein from Chinese habu (Trimeresurus mucrosquamatus) venom".THROMBOSIS AND HAEMOSTASIS 91.6(2004):1168-1176. |
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| 2016110703.pdf（1658KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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