KMS KUNMING INSTITUTE OF ZOOLOGY.CAS
| Purification, characterization and cytokine release function of a novel Arg-49 phospholipase A(2) from the venom of Protobothrops mucrosquamatus | |
| Wei JF1,3; Li T1; Wei XL1; Sun QY2; Yang FM2; Chen QY1; Wang WY4; Xiong YL4; He SH*1,3; shoahenghe@hotmail.com | |
| 2006 | |
| 发表期刊 | BIOCHIMIE
 |
| ISSN | 0300-9084 |
| 卷号 | 88期号:10页码:1331-1342 |
| 合作性质 | 其它 |
| 摘要 | Group IIA phospholipase A(2) (PLA(2)) are major components in Viperidae/Crotalidae venom. In the present study, a novel PLA(2) named promutoxin with Arg at the site 49 has been purified from the venom of Protobothrops muerosquamatus by chromatography. It consists of 122 amino acid residues with a molecular mass of 13,656 Da assessed by MALDI-TOF. It has the structural features of snake venom group IIA PLA(2)s, but has no PLA2 enzymatic activity. Promutoxin shows higher amino acid sequence identity to the K49 PLA(2)S (72-95%) than to D49 PLA(2)S (52-58%). Promutoxin exhibits potent myotoxicity in the animal model with as little as 1 mu g of promutoxin causing myonecrosis and myoedema in the gastrocnemius muscle of mice. Promutoxin is also able to stimulate the release of IL-12, TNF alpha, IL-6 and IL-1 beta from human monocytes, and induce IL-2, TNF alpha and IL-6 release from T cells, indicating that this snake venom group IIA PLA2 is actively involved in the inflammatory process in man caused by snake venom poisoning. |
| 关键词 | Phospholipase a(2) Protobothrops Mucrosquamatu Myotoxicity Monocytes t Cells |
| 资助者 | This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China (973 Pro- gram) (No. 2001CB510009); the National Natural Science Foundation of China (No. 30471601, 30570813); the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/2813 |
| 专题 | 其他 |
| 通讯作者 | shoahenghe@hotmail.com |
| 作者单位 | 1.Allergy and Inflammation Research Institute, The Shantou University Medical College, Xinling Road 11, 515031 Shantou, Guangdong, China 2.The Key Laboratory of Chemistry for Natural Products, Guizhou Province and Chinese Academy of Sciences, 550002 Guiyang, GuiZhou, China 3.First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China 4.Department of Animal Toxicology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 650223 Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Wei JF,Li T,Wei XL,et al. Purification, characterization and cytokine release function of a novel Arg-49 phospholipase A(2) from the venom of Protobothrops mucrosquamatus[J]. BIOCHIMIE,2006,88(10):1331-1342. |
| APA | Wei JF.,Li T.,Wei XL.,Sun QY.,Yang FM.,...&shoahenghe@hotmail.com.(2006).Purification, characterization and cytokine release function of a novel Arg-49 phospholipase A(2) from the venom of Protobothrops mucrosquamatus.BIOCHIMIE,88(10),1331-1342. |
| MLA | Wei JF,et al."Purification, characterization and cytokine release function of a novel Arg-49 phospholipase A(2) from the venom of Protobothrops mucrosquamatus".BIOCHIMIE 88.10(2006):1331-1342. |
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