N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)

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	N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)
	Wei JF 1,2; Wei XL 1; Chen QY 1; Huang T 1; Qiao LY 1; Wang WY 2; Xiong YL 2; He SH*1; shoahenghe@hotmail.com
	2006
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN	0304-4165
卷号	1760 期号:3 页码:462-471
合作性质	其它
摘要	A novel phospholipase A(2) (PLA(2)) with Asn at its site 49 was purified from the snake venom of Protobothrops mucrosquamatus by using SP-Sephadex C25, Superdex 75, Heparin-Sepharose (FF) and HPLC reverse-phage C-18 chromatography and designated as TM-N49. It showed a molecular mass of 13.875 kDa on MALDI-TOF. TM-N49 does not possess enzymatic, hemolytic and hemorrhagic activities. It fails to induce platelet aggregation by itself, and does not inhibit the platelet aggregation induced by ADP. However, it exhibits potent myotoxic activity causing inflammatory cell infiltration, severe myoedema, myonecrosis and myolysis in the gastrocnemius muscles of BALB/c mice. Phylogenetic analysis found that that TM-N49 combined with two phospholipase A(2)s from Trimeresurus stejnegeri, TsR6 and CTs-R6 cluster into one group. Structural and functional analysis indicated that these phospholipase A(2)s are distinct from the other subgroups (D49 PLA(2), S49 PLA(2) and K49 PLA(2)) and represent a unique subgroup of snake venom group II PLA(2), named N49 PLA(2) subgroup.
关键词	Molecular Cloning Phylogenetic Analysis Myotoxicity Phospholipase a(2) Snake Venom Protobothrops Mucrosquamatus
资助者	This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009).
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收录类别	SCI
语种	英语
资助者	This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001) and the Major State Basic Research Program of China (973 Program) (No. 2001CB510009).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2815
专题	其他
通讯作者	shoahenghe@hotmail.com
作者单位	1.Allergy and Inflammation Research Institute, the Shantou University Medical College, Shantou, Guangdong, 515031, China 2.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Wei JF,Wei XL,Chen QY,et al. N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2006,1760(3):462-471.
APA	Wei JF.,Wei XL.,Chen QY.,Huang T.,Qiao LY.,...&shoahenghe@hotmail.com.(2006).N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2).BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1760(3),462-471.
MLA	Wei JF,et al."N49 phospholipase A(2), a unique subgroup of snake venom group II phospholipase A(2)".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1760.3(2006):462-471.

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