Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase

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	Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase
	Wei JF 1,2; Mo YZ 1; Qiao LY 1; Wei XL 1; Chen HQ 1; Xie H 1; Fu YL 1; Wang WY 2; Xiong YL 2; He SH[*]1; weijifu@hotmail.com ; shoahenghe@hotmail.com
	2006
发表期刊	INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
ISSN	1357-2725
卷号	38 期号:4 页码:510-520
合作性质	其它
摘要	Poisonous snakebite wound is a popular disease worldwide. However, the pathogenesis remains unclear. In the present study, a novel metalloproteinase atrahagin in Chinese cobra (Naja atra) snake venom was purified, using heparin-sepharose followed by Superdex 75 gel filtration chromatography. Apart from its alpha-fibrinogenase activity, atrahagin potently activated human colon, lung and tonsil mast cells with the net histamine release being 25.9 +/- 4.4, 17.0 +/- 1.9, 13.2 +/- 3.6%, respectively. Time course studies revealed that the peak histamine release induced by atrahagin occurred at 12, 12 and 8 min following incubation of the enzyme with colon, lung and tonsil mast cells, respectively. The response of mast cells to atrahagin was abolished by preincubation of the cells with metabolic inhibitors or pertussis toxin, and by removal of Ca2+ and Mg2+ from the challenge buffer. In conclusion, activation of human mast cells by atrahagin indicated that the enzyme might contribute to the pathogenesis of snakebite wound.
关键词	Histamine Metalloproteinase Mast Cell Naja Atra G-protein Coupled Receptors
资助者	This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122).
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收录类别	SCI
语种	英语
资助者	This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122). ; This project was supported by the grants from the Li Ka Shing Foundation, Hong Kong, China (No. C0200001); the Major State Basic Research Program of China(973Program)(No.2001CB510009); the National Natural Science Foundation of China (No. 30471601);the Planned Science and Technology Project of Guang Dong Province, China (No. 2003B31502) and the National Natural Science Foundation of Guang Dong Province, China (No. 04106122).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2817
专题	其他
通讯作者	weijifu@hotmail.com; shoahenghe@hotmail.com
作者单位	1.Allergy and Inflammation Research Institute, The Shantou University Medical College, Shantou, Guangdong Province 515041, China 2.Department of Animal Toxicology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Wei JF,Mo YZ,Qiao LY,et al. Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2006,38(4):510-520.
APA	Wei JF.,Mo YZ.,Qiao LY.,Wei XL.,Chen HQ.,...&shoahenghe@hotmail.com.(2006).Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,38(4),510-520.
MLA	Wei JF,et al."Potent histamine-releasing activity of atrahagin, a novel snake venom metalloproteinase".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 38.4(2006):510-520.

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