A unique group of inactive serine protease homologues from snake venom

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	A unique group of inactive serine protease homologues from snake venom
	Wu JB 1,2; Jin Y 1; Zhong SR 3; Chen RQ 1; Zhu SM 1; Wang WY 1; Lu QM*1; Xiong YL 1; lvqm@mail.kiz.ac.cn
	2008
发表期刊	TOXICON
ISSN	0041-0101
卷号	52 期号:2 页码:277-284
摘要	A number of inactive serine protease homologues (SPHs), which have poorly understood functions, have been identified in invertebrates and vertebrates. Recently, several SPH transcripts have been reported from snake venom glands, which provide potential new tools for the study of the functions of SPHs. Herein we report for the first time a snake venom serine protease homologue (svSPH) protein, designated as TjsvSPH, isolated from the venom of Tilmeresurus jerdonii. Despite its high sequence similarity to snake venom serine proteases (SVSPs), TjsvSPH is devoid of arginine esterase and proteolytic activity. This is probably due to the replacement of Arg-43 by His-43 in the catalytic triad. TjsvSPH did not influence the coagulation time of human plasma, induce human platelet aggregation, inhibit adenosine diphosphate/thrombin-induced human platelet aggregation or increase capillary permeability. Phylogenetic analysis showed that svSPHs were separated from SVSPs and formed an independent group. Structural analysis revealed that the structures of svSPHs are quite different from those of SPHs previously reported. These results indicate that snake venoms contain a unique group of svSPH proteins. (C) 2008 Elsevier Ltd. All rights reserved.
关键词	Snake Venom Serine Protease Homologue Phylogenetic Analysis Trimeresurus Jerdonii
资助者	This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M).
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收录类别	SCI
语种	英语
资助者	This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). ; This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2821
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	lvqm@mail.kiz.ac.cn
作者单位	1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiaochang East Road, Kunming 650223, PR China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100080, PR China 3.Department of Forensic Biology, Faculty of Forensic Medicine, Kunming Medical College, Kunming 650031, PR China
推荐引用方式 GB/T 7714	Wu JB,Jin Y,Zhong SR,et al. A unique group of inactive serine protease homologues from snake venom[J]. TOXICON,2008,52(2):277-284.
APA	Wu JB.,Jin Y.,Zhong SR.,Chen RQ.,Zhu SM.,...&lvqm@mail.kiz.ac.cn.(2008).A unique group of inactive serine protease homologues from snake venom.TOXICON,52(2),277-284.
MLA	Wu JB,et al."A unique group of inactive serine protease homologues from snake venom".TOXICON 52.2(2008):277-284.

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