L-amino acid oxidase from Naja atra venom activates and binds to human platelets

	L-amino acid oxidase from Naja atra venom activates and binds to human platelets
	Li R 1; Zhu SW 1; Wu JB 1; Wang WY 1; Lu QM*1; Clemetson KJ 2; lvqm@mail.kiz.ac.cn
	2008
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	40 期号:1 页码:19-26
合作性质	其它
摘要	An L-amino acid oxidase (LAAO), NA-LAAO, was purified from the venom of Naja atra. Its N-terminal sequence shows great similarity with LAAOs from other snake venoms. NA-LAAO dose-dependently induced aggregation of washed human platelets. However, it had no activity on platelets in platelet-rich plasma. A low concentration of NA-LAAO greatly promoted the effect of hydrogen peroxide, whereas hydrogen per oxide itself had little activation effect on plate lets. NA-LAAO induced tyrosine phosphorylation of a number of platelet proteins including Src kinase, spleen tyrosine kinase, and phospholipase C gamma 2. Unlike convulxin, Fc receptor gamma chain and T lymphocyte adapter protein are not phosphorylated in NA-LAAO-activated platelets, suggesting an activation mechanism different from the glycoprotein VI pathway. Catalase inhibited the platelet aggregation and platelet protein phosphorylation induced by NA-LAAO. NA-LAAO bound to fixed platelets as well as to platelet lysates of Western blots. Furthermore, affinity chromatography of platelet proteins on an NA-LAAO-Sepharose 4B column isolated a few platelet membrane proteins, suggesting that binding of NA-LAAO to the platelet membrane might play a role in its action on platelets.
关键词	L-amino Acid Oxidase Naja Atra Platelet Hydrogen Peroxide
资助者	This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences ; This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/2823
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	lvqm@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Theodor Kocher Institute, University of Berne, Berne CH-3012, Switzerland
推荐引用方式 GB/T 7714	Li R,Zhu SW,Wu JB,et al. L-amino acid oxidase from Naja atra venom activates and binds to human platelets[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(1):19-26.
APA	Li R.,Zhu SW.,Wu JB.,Wang WY.,Lu QM*.,...&lvqm@mail.kiz.ac.cn.(2008).L-amino acid oxidase from Naja atra venom activates and binds to human platelets.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(1),19-26.
MLA	Li R,et al."L-amino acid oxidase from Naja atra venom activates and binds to human platelets".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.1(2008):19-26.

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