Islet-1 is required for ventral neuron survival in Xenopus

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	Islet-1 is required for ventral neuron survival in Xenopus
	Shi Y 1,2; Zhao SH 1,2; Li JJ 1,2; Mao BY*1; mao@mail.kiz.ac.cn
	2009
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	388 期号:3 页码:506-510
合作性质	其它
摘要	Islet-1 is a LIM domain transcription factor involved in several processes of embryonic development. Xenopus Islet-1 (Xisl-1) has been shown to be crucial for proper heart development. Here we show that Xisl-1 and Xisl-2 are differentially expressed in the nervous system in Xenopus embryos. Knock-down of Xisl-1 by specific morpholino leads to severe developmental defects, including eye and heart failure. Staining with the neuronal markers N-tubulin and Xisl-1 itself reveals that the motor neurons and a group of ventral interneurons are lost in the Xisl-1 morphants. Terminal dUTP nick-end labeling (TUNEL) analysis shows that Xisl-1 morpholino injection induces extensive apoptosis in the ventral neural plate, which can be largely inhibited by the apoptosis inhibitor M50054. We also find that over-expression of Xisl-1 is able to promote cell proliferation and induce Xstat3 expression in the injected side, suggesting a potential role for Xisl-1 in the regulation of cell proliferation in co-operation with the Jak-Stat pathway.
关键词	Islet-1 Islet-2 Xenopus Motoneuron Apoptosis Cell Proliferation Stat3
资助者	This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Na- tional Natural Science Foundation of China (30425011 and 305303800), the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201) and the Innovation Project of Chinese Academy of Sciences (KSCX2-YW-R-090).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3167
专题	科研部门_发育生物学（毛炳宇）遗传资源与进化国家重点实验室
通讯作者	mao@mail.kiz.ac.cn
作者单位	1.CAS-Max Planck Junior Scientist Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Shi Y,Zhao SH,Li JJ,et al. Islet-1 is required for ventral neuron survival in Xenopus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,388(3):506-510.
APA	Shi Y,Zhao SH,Li JJ,Mao BY*,&mao@mail.kiz.ac.cn.(2009).Islet-1 is required for ventral neuron survival in Xenopus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,388(3),506-510.
MLA	Shi Y,et al."Islet-1 is required for ventral neuron survival in Xenopus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 388.3(2009):506-510.

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