RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination

KIZ OpenIR > 科研部门 > 发育生物学（毛炳宇）

	RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination
	Kong QH 1,2; Zeng WL 1,2; Wu JY 1,2; Hu WL 1,2; Li CC 1; Mao BY[*]1; mao@mail.kiz.ac.cn
	2010
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	393 期号:4 页码:708-713
合作性质	其它
摘要	Modification of proteins by ubiquitination plays important roles in various cellular processes. During this process, the target specificity is determined by ubiquitin ligases. Here we identify RNF220 (RING finger protein 220) as a novel ubiquitin ligase for Sin3B. As a conserved RING protein, RNF220 can bind E2 and mediate auto-ubiquitination of itself. Through a yeast two-hybrid screen, we isolated Sin3B as one of its targets, which is a scaffold protein of the Sin3/HDAC (histone deacetylase) corepressor complex. RNF220 specifically interacts with Sin3B both in vitro and in vivo. Sin3B can be regulated by the ubiquitin-proteasome system. Co-expression of RNF220 promotes the ubiquitination and proteasomal degradation of Sin3B. Taken together, these results reveal a new mechanism for regulating the Sin3/HDAC complex.
关键词	Rnf220 Sin3b E3 Ligase Ubiquitination Degradation
资助者	This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090). ; This work was supported by grants from the Key State Research Program from the Ministry of Science and Technology of China (2006CB943902 and 2007CB947201), the National Natural Science Foundation of China (30425011 and 305303800), and the Innova- tion Project of Chinese Academy of Sciences (KSCX2-YW-R-090).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3171
专题	科研部门_发育生物学（毛炳宇）遗传资源与进化国家重点实验室
通讯作者	mao@mail.kiz.ac.cn
作者单位	1.CAS-Max Planck Junior Scientist Group on Developmental Biology, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang East Road, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Kong QH,Zeng WL,Wu JY,et al. RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,393(4):708-713.
APA	Kong QH.,Zeng WL.,Wu JY.,Hu WL.,Li CC.,...&mao@mail.kiz.ac.cn.(2010).RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,393(4),708-713.
MLA	Kong QH,et al."RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 393.4(2010):708-713.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
20103934708.pdf（497KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文