餐洗剂对小鼠骨髓细胞SCE形成和Salmonella typhimurium基因回复突变的影响

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	餐洗剂对小鼠骨髓细胞SCE形成和Salmonella typhimurium基因回复突变的影响
其他题名	Gentical effect of Surfactants on Sce Formation of Mouse Bone Marrow Cells and Gene Mutation of Salmonella Typhimurium
	胡嘉想; 林世英; 刘爱华; 张汉云
	1992
发表期刊	动物学研究
ISSN	0254-5853
卷号	13 期号:1 页码:83-88
摘要	本研究采用Salmonella typhimurium基因回复突变测试和小鼠骨髓细胞姐妹染色单体交换(SCE)分析,研究了四种餐洗剂及其主要成分LABS、Dispersol D、Alkanolamide的潜在诱变活性。结果表明四种牌号餐洗剂在高浓度时能诱发SCE形成,除TL牌餐洗剂加入S9代谢激活系统后未见诱变作用外,其他三种餐洗剂和三种主要成分都表现出致HisG46和HisD3052基因回复突变的能力。在相当于人日常使用量时,没观察出它们使SCE频率升高的现象,也不表现出致STY基因回复突变的能力。实验还观察了餐洗剂对小鼠骨髓细胞增殖的毒性效应,及其主要成分使细菌菌落异常生长的现象,并讨论了不同诱变效应之间可能存在的关系。
其他摘要	The research employing the techniques of Salmonella/microsome reversion assay and SCE analysis evaluates systematically the mutagenic potential of fourbrand household liquid detergents and three kinds of indegredients,LABS,Dispersol D and Alkanolamide,based on the effects of these surfactants on the STY test strains with the HisD3052 and HisG46 mutations and on the SCE formation of mouse bone marrow cells.These results indicate that,in the absence of metabolic activators,these four-brand detergents,and LABS and Dispersol D all can significantly reverse the mutation HisD3052 and HisG46 to histidine independence,compared with negative control.Akanolamide also can elicit the frameshift mutation HisD3052,a positive response,but a negative effect appears in the case of the missense mutation.Otherwise,although,when implemented with exogenous source of metabolic activation,detergent TL dose not exhibit positive response in STY bioassay for mutagenic activity,the other three kinds of detergents and their three main components,Dispersol D,Alkanolamide and LABS can induce a significant reproducible increase in number of the reversant colonies compared with negative control.Nevertheless,it is below certain doses of test materials,or an equivalent exposing to human beings during their house routine that all these test chemicals reproduce a negative response either with or without metabolic activation.The results of SCE analysis demonstrate that four-brand detergents,compared with the solvent control,can induce the SCE formation of mouse bone marrow cells.Their cytotoxicity is observed-that the rates of bone marrow cell proliferation decrease after oral administration of high-dose detergents,and highest doses of all these chemicals tested do give an indication of toxicity as evidenced by the presence of pin-point abnormal colonies on the plates,or the reduction in the spontaneous reversion rate.In addition,the possible relationship between different mutagenic responses is disscussed.
部门归属	其他
关键词	餐洗剂基因回复突变姐妹染色单体交换
URL	查看原文
收录类别	其他
语种	中文
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3219
专题	其他
作者单位	中国科学院昆明动物研究所,昆明650107
推荐引用方式 GB/T 7714	胡嘉想,林世英,刘爱华,等. 餐洗剂对小鼠骨髓细胞SCE形成和Salmonella typhimurium基因回复突变的影响[J]. 动物学研究,1992,13(1):83-88.
APA	胡嘉想,林世英,刘爱华,&张汉云.(1992).餐洗剂对小鼠骨髓细胞SCE形成和Salmonella typhimurium基因回复突变的影响.动物学研究,13(1),83-88.
MLA	胡嘉想,et al."餐洗剂对小鼠骨髓细胞SCE形成和Salmonella typhimurium基因回复突变的影响".动物学研究 13.1(1992):83-88.

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1992130183.pdf（289KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文