Methylation of tumor associated genes in tissue and plasma samples from liver disease patients

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	Methylation of tumor associated genes in tissue and plasma samples from liver disease patients
	Chang H 1,5; Yi B 1,5; Li L 2,5; Zhang HY 3; Sun F 4; Dong SQ 3; Cao Y*1,5; caoy@mail.kiz.ac.cn
	2008
发表期刊	EXPERIMENTAL AND MOLECULAR PATHOLOGY
ISSN	0014-4800
卷号	85 期号:2 页码:96-100
摘要	To investigate whether aberrant hypermethylation in plasma DNA could be used as diagnosis makers for hepatocellular carcinoma (HCC), we performed methylation-specific PCR (MSP) to check the methylation status of five tumor associated genes in 36 cases of tissue and 42 cases of plasma samples from HCC and liver cirrhosis patients, respectively. The hypermethylation frequency of GSTP1 and RASSF1A showed significant difference between HCCs and liver cirrhosis with OF without HBV infection (P < 0.05), but differences of the hypermethylation status of APC, E-cadherin, and P16 were not statistically significant. There were no significant differences in the hypermethylation status of five genes between the groups of cirrhosis with and without HBV infection. The significant differences of E-cadherin, GSTP1, P16, and RASSF1A in methylation between HCCs and liver cirrhosis were not observed in the plasma samples. Furthermore, the inconsistent results of MSP and real-time quantitative PCR for the paired samples of tissue and plasma suggested that plasma DNA could not fully stand for tissue DNA. In conclusion, hypermethylation of some specific, but not all, tumor associated genes may be involved in hepatocarcinogenesis; examination of the methylation status of E-cadherin, GSTP1, P16, and RASSF1A in the plasma samples might have limited usage for HCC diagnosis.
关键词	Hepatocellular Carcinoma Cirrhosis Plasma Dna Hypermethylation Methylation-specific Pcr
资助者	This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M).
收录类别	SCI
语种	英语
资助者	This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M). ; This study was supported by a grant from Yunnan Province Science and Technology Department (2007C097 M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3369
专题	分子病理学科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	caoy@mail.kiz.ac.cn
作者单位	1.Laboratory of Molecular and Experimental Pathology, Key Laboratory of Animal Models and Human Disease Mechanism, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Department of General Surgery, Kunming First People Hospital, Kunming, China 3.Division of Digestive Diseases, Kunming First People Hospital, Kunming, China 4.Surgical Department of Hepato-Bilio-Pancreatology, Second Affiliated Hospital of Kunming Medical Collage, Kunming, China 5.Yunnan Liver Diseases Center, Kunming, China
推荐引用方式 GB/T 7714	Chang H,Yi B,Li L,et al. Methylation of tumor associated genes in tissue and plasma samples from liver disease patients[J]. EXPERIMENTAL AND MOLECULAR PATHOLOGY,2008,85(2):96-100.
APA	Chang H.,Yi B.,Li L.,Zhang HY.,Sun F.,...&caoy@mail.kiz.ac.cn.(2008).Methylation of tumor associated genes in tissue and plasma samples from liver disease patients.EXPERIMENTAL AND MOLECULAR PATHOLOGY,85(2),96-100.
MLA	Chang H,et al."Methylation of tumor associated genes in tissue and plasma samples from liver disease patients".EXPERIMENTAL AND MOLECULAR PATHOLOGY 85.2(2008):96-100.

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