| Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells | |
| Cao Y1,2; Merling A1; Karsten U3,4; Goletz S4; Punzel M5; Kraft R3; Butschak G3; Schwartz-Albiez R*1; r.s-albiez@dkfz.de | |
| 2008 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF CANCER
 |
| ISSN | 0020-7136 |
| 卷号 | 123期号:1页码:89-99 |
| 合作性质 | 其它 |
| 摘要 | The expression of the histo-blood group carbohydrate structures T-nouvelle (Tn, CD175), sialylated To (CD175s) and the Thomsen-Friedenreich disaccharide (TF, CD176) on human leukemia cell lines was analyzed by their reactivity with specific monoclonal antibodies in flow cytometry, immunohistology and immunoprecipitation. Expression of sialylated CD176 was evaluated by comparative immunostaining with anti-CD176 antibodies before and after sialidase treatment. While only few cell lines expressed unmasked CD176, sialylated CD176 was present on all hematopoietic cell lines and native lymphocytes examined. CD175 and CD175s are preferentially expressed on erythroblastic leukemia cell lines. CD175s expression in these cells is consistent with the transcription of the gene encoding the key enzyme alpha 2,6-sialyltransferase (hST6GalNAc1). The staining intensity was reduced after methanol pretreatment of cells, indicating that these glycans are partially expressed as constituents of glycosphingolipids. Immunoprecipitation and subsequent Western blotting revealed a series of distinct high molecular glycoproteins as carriers for these carbohydrate antigens. CD34 was identified as major carrier of CD176 by immunoprecipitation and microsequencing on a KG-1 subline enriched for CD176 expression. Incubation of several CD176-positive cell lines with anti-CD176 antibodies induced apoptosis of these cells, an effect not observed with anti-CD175/CD175s antibodies. Since the presence of naturally occurring anti-CD176 antibodies may represent a mechanism of immunosurveillance against CD176-positive tumor cells, we propose that sialylation of surface-expressed CD176-among other functions-protects against apoptosis. |
| 关键词 | Thomsen-friedenreich-related Antigens (Tfra) Monoclonal Antibodies Cd175 Cd176 Leukemia Cells Apoptosis Cd34 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/3373 |
| 专题 | 分子病理学 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | r.s-albiez@dkfz.de |
| 作者单位 | 1.Division of Cellular Immunology, German Cancer Research Center, Heidelberg, Germany 2.Laboratory of Molecular and Experimental Pathology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 3.Max Delbrcuck Center for Molecular Medicine, Berlin-Buch, Germany 4.Glycotope GmbH, Berlin-Buch, Germany 5.Institute for Transfusion Medicine, University Hospital, University D€ usseldorf, Germany |
| 推荐引用方式 GB/T 7714 | Cao Y,Merling A,Karsten U,et al. Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells[J]. INTERNATIONAL JOURNAL OF CANCER,2008,123(1):89-99. |
| APA | Cao Y.,Merling A.,Karsten U.,Goletz S.,Punzel M.,...&r.s-albiez@dkfz.de.(2008).Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells.INTERNATIONAL JOURNAL OF CANCER,123(1),89-99. |
| MLA | Cao Y,et al."Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells".INTERNATIONAL JOURNAL OF CANCER 123.1(2008):89-99. |
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| 200812389.pdf（479KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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