蓝氏贾第虫核被膜缺口的电镜观察

	蓝氏贾第虫核被膜缺口的电镜观察
其他题名	Electron-Microscopical Observations on the Nuclear Envelope Openings of Giardia lamblia
	沈剑钊 1; 李靖炎 2; 卢思奇 1
	1996
发表期刊	动物学研究
ISSN	0254-5853
卷号	17 期号:3 页码:291-294
摘要	以蓝氏贾第虫作为双滴虫类的代表, 是迄今所知的现存最原始的真核生物类群。对其细胞核进行了电镜观察。除未见核仁外, 还发现其核被膜的横切面上存在有缺口, 在缺口的边缘处, 核内膜与核外膜是相互连接的。核被膜缺口处常有一核纤层样的薄层分隔核质与细胞质。核被膜缺口的发现证实了李靖炎(1979)的核被膜起源假说的推断。
其他摘要	There are evidences showing that diplomonads would be the most primitive eukaryotes among all present-existing organisms.Therefore,the informations about diplomonads nucleus are very important for investigating the origin and early evolution of cell nucleus.However,they are very seldom in literature.The nucleus of Giardia lamblia was investigated as a representative of diplomonad nucleus with electron-microscopy.The perinuclear space within the nuclear envelope of Giardia is very prominent and on this background we found that there were openings on the nuclear envelope,which were very much larger than the nuclear pores.The sixe of the opening varied in different nuclei.At the margin of the opening.the nuclear inner membrane is continuous with the nuclear outer membrane.Therefore,the opening can not be the artefact due to sectioning.Usually,at the opening,there is a nuclear lamina-like thin layer separating nucleoplasm from cytoplasm.The layer seems not membranous,because when Giardia cells were fixed with KMnO[4] to preserve only membranous structures,this layer were absent.The layer might perhaps be a part of the nuclear lamina of Giardia.Although after a series of specific extractions to demonstrate Giardia nuclear matrix;nuclear lamina could not be found (Dai et al.,1996),it might still be exist but only so primitive that could not bear the routine extrations.Nuclear envelope must have originated from the primitive endoplasmic reticulum in the closest prokaryotic ancestor of eukaryotic cells (Li,1979).Those parts of the primitive endoplasmic reticulum,which were directly neighboring to the"nuclear region"of the prokaryotic ancestor evolved into nuclear envelope (Li,1996).According to this hypothesis,at the earlier stage of this evolutionary process,the newly formed primitive nuclear envelope could not enclose the whole nuclear region and there must have remained many openings.The discovery of nuclear envelope openings in Giardia nucleus has confirmed this deduction.
部门归属	其他
关键词	蓝氏贾第虫核被膜缺口核被膜的起源
资助者	国家自然科学基金 ; 国家自然科学基金
URL	查看原文
收录类别	其他
语种	中文
资助者	国家自然科学基金 ; 国家自然科学基金
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3449
专题	科研部门_真核细胞进化基因组（文建凡）细胞与分子进化开放实验室
作者单位	1.首都医科大学寄生虫学教研室 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223
推荐引用方式 GB/T 7714	沈剑钊,李靖炎,卢思奇. 蓝氏贾第虫核被膜缺口的电镜观察[J]. 动物学研究,1996,17(3):291-294.
APA	沈剑钊,李靖炎,&卢思奇.(1996).蓝氏贾第虫核被膜缺口的电镜观察.动物学研究,17(3),291-294.
MLA	沈剑钊,et al."蓝氏贾第虫核被膜缺口的电镜观察".动物学研究 17.3(1996):291-294.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1996173291.pdf（262KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文