涡鞭毛虫(甲藻)着丝粒/动粒蛋白的检索

KIZ OpenIR > 其他

	涡鞭毛虫(甲藻)着丝粒/动粒蛋白的检索
其他题名	The Detection of Centromere/Kinetochore Proteins in Typical Dinoflagellate and Oxyrrhis
	吴传芬 1; 李靖炎*2; 代嘉陵 2; 王永潮 1
	1996
发表期刊	动物学研究
ISSN	0254-5853
卷号	17 期号:3 页码:307-313
摘要	用ACA血清作荧光观察, 隐沟虫细胞动粒蛋白表明, 隐沟虫的这些蛋白虽结合在核骨架上, 但在间期时并不形成点状的前着丝粒。免疫印迹检查表明隐沟虫和尖尾虫的着丝粒蛋白B彼此一致, 而且与四膜虫和眼虫也高度一致。但用ACA血清作免疫印迹检查时, 尖尾虫的蛋白虽与四膜虫和眼虫的相近, 与隐沟虫的却有极大的差异。以抗动粒蛋白的单抗作此种检查时, 尖尾虫与眼虫的反应带相同, 而隐沟虫则与源真核生物(Archezoa)贾第虫(Giardia lamblia)的相同; 而且隐沟虫和贾第虫都与几种原细菌有两条相同的反应带。
其他摘要	When typical dinoflagellate,Crypthecodinium cohnii,was detected with immuno-fluorescent microscopy,its nucleus gave positive reaction to ACA serum against human centromere/kinetochore proteins.However,in its nucleus,just as in nuclei of Tetrahymena and Euglena,spot-like precentromeres have never been found.The immuno-blotting detections with the monoclonal antibody,mACA-2,against human centromere protein,CENP-B,showed that Crypthecodinium and the special dinoflagellate,Oxyrrhis marina,gave the same result which was correspondent with those given by Tetrahymena,Euglena and human Hep-2 cells.With the CENP-B polyclonal antibody,ra-ACA-2,the two dinoflagellates,other protists and Hep-2 cells also gave the results identical to each.These results indicated that CENP-B was highly conservative in eukaryotic evolution.In fact,all the centromere/kinetochore proteins are quite conservative.Therefore,in the immuno-blotting detections done with ACA serum,the positive bands of Tetrahymena and Euglena are quite similar to those of human Hep-2 cells.The bands of the special dinoflagellate,Oxyrrhis,are similar to other protists.although a few bands are absent.However,the bands of typical dinoflagellate are obviously different from Oxyrrhis Sand other eukaryotes.It means that two dinoflagellates are far from each other.The results obtained with the monoclonal antibody,mAb37A5,against the kinetochore proteins of CHO cells,also proposed the above suggestion.In the detections,Tetrahymena gave only a 120 kD band; Euglena and Oxyrrhis gave two bands (45 kD and 120 kD),while Crypthecodinium and archezoan,Giardia,gave three bands (45 kD,50 kD and 120 kD).It is very interesting that various archaebacteria all gave the 45 kD band and the 50 kD band (the third band is of 40 kD)(Wu et al.,1996b).
部门归属	其他
关键词	涡鞭毛虫（甲藻）的分类隐沟虫尖昆虫着丝粒／动粒蛋白免疫印迹
URL	查看原文
收录类别	其他
语种	中文
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3453
专题	其他细胞与分子进化开放实验室
作者单位	1.北京师范大学生物系,北京1000875 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223
推荐引用方式 GB/T 7714	吴传芬,李靖炎*,代嘉陵,等. 涡鞭毛虫(甲藻)着丝粒/动粒蛋白的检索[J]. 动物学研究,1996,17(3):307-313.
APA	吴传芬,李靖炎*,代嘉陵,&王永潮.(1996).涡鞭毛虫(甲藻)着丝粒/动粒蛋白的检索.动物学研究,17(3),307-313.
MLA	吴传芬,et al."涡鞭毛虫(甲藻)着丝粒/动粒蛋白的检索".动物学研究 17.3(1996):307-313.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1996173307.pdf（688KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文