小眼虫的着丝粒蛋白检查

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	小眼虫的着丝粒蛋白检查
其他题名	The Detection of Centromere Proteins in Guglena gracilis
	吴传芬 1; 代嘉陵 2; 李靖炎 2; 王永潮 1
	1996
发表期刊	动物学研究
ISSN	0254-5853
卷号	17 期号:4 页码:501-507
摘要	为了从起源与进化的角度考察着丝粒蛋白, 作者从比酵母更低等的原生生物着手, 检查它们的着丝粒蛋白. 另文作者已报道了对四膜虫研究的结果, 该文报道的是对小眼虫(Englena gracilis) 检查. 作者的结果表明: 眼虫细胞核里存在的 ACA 抗原的分子量, 包括了用 ACA 血清在高等生物中检出来的 CENP-A、CENP-B、CENP-C 和 CENP-C 这几类基本的着丝粒蛋白组分. 作免疫荧光染色后, 细胞核呈很强的阳性反应, 但分辨不出前着丝粒小点. 核仁不被荧光染色, 除了核仁外似乎整个核都被染色. 这可能反映了抗原的分布在核内不是集中成点状的. 经过制备核骨架的程序处理后, 细胞核仍为阳性(尽管荧光较弱). 如此看来, 眼虫至少已有相当大一部分的 ACA 抗原是与核骨架结合着的. 对分离的细胞核作相应的抽提处理后再作免疫印迹检查, 得到的阳性反应带也证明了这一点。
其他摘要	In this paper we report our detection of centromere proteins in Euglena gracilis.With Immunofluorescent microscopy stained with Chinese ACA antiserum,in the interphase nucleus of HepⅡ cells (human throat cancer cells) only very minute pots—precentromere could be observed.When Euglena was stained with the same technique,there were only patched within nuclei composed of minute fluorescent spots;no precentromere could be distinguished,although all the nuclei gave positive reaction.Perhaps the distribution of centromere kinetochore proteins within the interphase nucleus of protists was different from that of higher organisms.After sequential selective extractions to prepare nuclerar matrix,the nuclei of Euglena still gave reactions to prepare nuclear matrix,the nuclei of Euglena still gave reaction to ACA serum under immunofluorescent microscope.This fact indicates that the centromere/kinetochore proteins of protists are also tightly combined with nuclear matrix,just as those of higher organisms do.This tight combination with nuclaer matrix was further proved with immnoblotting.The positive band-pattern of Euglena cells was still similar to that of HepⅡ after immunoblotting with Chinese ACA serum,although the band number was slightly fewer.The molecular weights of positive bands indicate that euglenoid has likely possessed the main components of centromere proteins; CENP-A,CENP-B,CENP-C and CENP-D etc.The blotting results with Maca-2 antibody showed that HepⅡ cells gave two bands (80 Kd,stained deely and 120 Kd,stained slightly) Euglena gave three bands 80 Kd,120 Kd,and 150 Kd.The blotting results with ra-ACA-2 antiserum were some what different,although it also recognized human CENP-B protein.All HepⅡ and Euglena gave 50 Kd,60 Kd and 80 Kd band.In the immunoblotting with monoclonal antibody mAb37A5,HepⅡ cells gave two bands very similar to those of CHO cells.Euglena showed two bands,one identical to the tetrahymena band,another being about 55 Kd.
部门归属	其他
关键词	小眼虫着丝粒蛋白 Aca血清
URL	查看原文
收录类别	其他
语种	中文
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3493
专题	其他细胞与分子进化开放实验室
作者单位	1.北京师范大学生物系,北京1000875 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223
推荐引用方式 GB/T 7714	吴传芬,代嘉陵,李靖炎,等. 小眼虫的着丝粒蛋白检查[J]. 动物学研究,1996,17(4):501-507.
APA	吴传芬,代嘉陵,李靖炎,&王永潮.(1996).小眼虫的着丝粒蛋白检查.动物学研究,17(4),501-507.
MLA	吴传芬,et al."小眼虫的着丝粒蛋白检查".动物学研究 17.4(1996):501-507.

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1996174501.pdf（724KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文