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On the nucleoli of the dinoflagellate Prorocentrum
Li JY
1985
发表期刊Hydrobiologia
卷号124期号:1页码:45-48
合作性质其它
摘要To date no nucleolus had been observed in Prorocentrum under the light microscope. The author failed to show the nucleoli of P. micans and P. cassubica with eosin in 70% alc or with methyl green-pyronin. But when these dinoflagellates were treated with an Ag-1 technique which had been improved for demonstrating NORs in unicellular organisms, nucleoli were stained dark brown or black, while all other parts showed no colour. When the materials were stained well, only the central part of the nucleolus was stained. Under the electron microscope, it was observed that all the silver grains were concentrated in the pars fibrosa of the nucleolus. P. cassubica had only one small oblate nucleolus attached to the nuclear envelope, with NOR usually in the shape of the letters O or C. P. micans had 1–7 nucleoli of various sizes and shapes with NORs in various complicated forms. The number of nucleoli bore a certain relationship to the living state of the dinoflagellate. One day after fresh medium was added, cells with 3 nucleoli were most common, and 28.5% of the individuals had 4–6 nucleoli. Cells having only one nucleolus accounted for 8.6%. 3 days after, cells with 2 nucleoli became dominant, and those with 4–6 decreased to 18.4%. After a month, cells with 1 nucleolus became most abundant, cells having 4 nucleoli decreased to 2.4%, and no cells had 5 or 6 nucleoli.
关键词Nucleolus Dinoflagellate Selver-staining Culture
收录类别其他
语种英语
文献类型期刊论文
条目标识符http://ir.kiz.ac.cn/handle/152453/366
专题其他
作者单位Evolutionary Cell Biology LaboratoryKunming Institute of ZoologyKunming, YunnanChina
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Li JY. On the nucleoli of the dinoflagellate Prorocentrum[J]. Hydrobiologia,1985,124(1):45-48.
APA Li JY.(1985).On the nucleoli of the dinoflagellate Prorocentrum.Hydrobiologia,124(1),45-48.
MLA Li JY."On the nucleoli of the dinoflagellate Prorocentrum".Hydrobiologia 124.1(1985):45-48.
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