Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

	Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda
	Ding B 1; Shi P1 ; Xiangyu JG 1; Zhang YP*1; Chen DY 2; Sun QY 2; Li GP 2; Wang MK 2; Liu YL 2; Kang L 2; Han ZM 2; Song XF 2; Li JS 2; Chen YC 3
	2000
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	45 期号:20 页码:1883-1885
合作性质	其它
摘要	A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g(010) was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.
关键词	Giant Panda (Ailuropoda Melanoleuca) Interspecies Cloning Microsatellite Dna Nucleus
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收录类别	SCIE
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/3963
专题	科研部门_分子进化与基因组多样性（张亚平）遗传资源与进化国家重点实验室科研部门_进化与功能基因组学（施鹏）细胞与分子进化开放实验室
作者单位	1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences. Kunming 650223, China 2.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 3.Fuzhou Panda Institute Center, Fuzhou 350001, China
推荐引用方式 GB/T 7714	Ding B,Shi P,Xiangyu JG,et al. Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda[J]. CHINESE SCIENCE BULLETIN,2000,45(20):1883-1885.
APA	Ding B.,Shi P.,Xiangyu JG.,Zhang YP*.,Chen DY.,...&Chen YC.(2000).Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda.CHINESE SCIENCE BULLETIN,45(20),1883-1885.
MLA	Ding B,et al."Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda".CHINESE SCIENCE BULLETIN 45.20(2000):1883-1885.

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