Apoptotic cells induce immunosuppression through dendritic cells: Critical roles of IFN-gamma and nitric oxide

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	Apoptotic cells induce immunosuppression through dendritic cells: Critical roles of IFN-gamma and nitric oxide
	Ren GW 1; Su JJ 2; Zhao X 1; Zhang LY 1; Zhang JM 1; Roberts AI 1; Zhang HT 2; Das G 1; Shi YF*1,2; shiyu@umdnj.edu
	2008
发表期刊	JOURNAL OF IMMUNOLOGY
ISSN	0022-1767
卷号	181 期号:5 页码:3277-3284
合作性质	其它
摘要	Apoptotic cells induce immunosuppression through unknown mechanisms. To identify the underlying molecular mediators, we examined how apoptotic cells induce immunoregulation by dendritic cells (DC). We found that administration of DC exposed to apoptotic cells (DCap) strongly inhibited the expansion of lymphocytes in draining lymph nodes in vivo and the subsequent Ag-specific activation of these lymphocytes ex vivo. Unexpectedly, Map supported T cell activation to a similar extent as normal DC in vitro, leading to proliferation and IL-2 production, except that DCap did not support T cell production of IFN-gamma. Surprisingly, when DCap were cocultured with normal DC, they completely lost their ability to support T cell activation, an effect reversed by anti-IFN-gamma or inhibitors of inducible NO synthase (iNOS). As expected, exposure to apoptotic cells rendered DC.,, capable of producing much more NO in response to exogenous IFN-gamma than normal DC. Furthermore, DCap from iNOS(-/-) or IFN-gamma RI-/- mice were not inhibitory in vitro or in vivo. Therefore, the IFN-gamma-induced production of NO by apoptotic cell-sensitized DC plays a key role in apoptotic cell-mediated immunosuppression.
资助者	This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported
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收录类别	SCI
语种	英语
资助者	This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported ; This work was supported in part by New Jersey Commission on Science and Technology (NJCST-2042-014-84), United States Public Health Service Grants (AI057596 and AI50222), and the National Space Biomedical Research Institute (IIH00405), which is supported
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4431
专题	分子免疫生物学
通讯作者	shiyu@umdnj.edu
作者单位	1.Department of Molecular Genetics, Microbiology and Immunology, Robert Wood John- son Medical School-University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854 2.School of Life Science, University of Science and Technology of China and Overseas Immunobiology Team, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China
推荐引用方式 GB/T 7714	Ren GW,Su JJ,Zhao X,et al. Apoptotic cells induce immunosuppression through dendritic cells: Critical roles of IFN-gamma and nitric oxide[J]. JOURNAL OF IMMUNOLOGY,2008,181(5):3277-3284.
APA	Ren GW.,Su JJ.,Zhao X.,Zhang LY.,Zhang JM.,...&shiyu@umdnj.edu.(2008).Apoptotic cells induce immunosuppression through dendritic cells: Critical roles of IFN-gamma and nitric oxide.JOURNAL OF IMMUNOLOGY,181(5),3277-3284.
MLA	Ren GW,et al."Apoptotic cells induce immunosuppression through dendritic cells: Critical roles of IFN-gamma and nitric oxide".JOURNAL OF IMMUNOLOGY 181.5(2008):3277-3284.

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