| A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells | |
| Zhang P1,2; Li DM*3; Stewart-Jones G3; Shao XL1; Zhang YX2; Chen QY3; Li YJ1; He YW1,4; Xu XN*1,3; Zhang HT*1; demin.li@imm.ox.ac.uk | |
| 2009 | |
| 发表期刊 | IMMUNOLOGY
 |
| ISSN | 0019-2805 |
| 卷号 | 128期号:4页码:500-510 |
| 合作性质 | 其它 |
| 摘要 | The non-classical major histocompatibility complex (MHC) class I molecule CD1d presents lipid antigens to invariant natural killer T (iNKT) cells, which are an important part of the innate immune system. CD1d/iNKT systems are highly conserved in evolution, and cross-species reactivity has been suggested to be a common feature of different animals based on research in humans and mice. However, we found that CD1d from the tree shrew (Tupaia belangeri), a close evolutionary relative of primates, failed to stimulate human iNKT cells, despite being more homologous to human CD1d than that of mouse. Sequence comparison and molecular modelling showed that two of the key amino acid residues in human CD1d proposed to be in direct contact with T-cell receptors were mutated in tree shrew CD1d. Substitution of one of the residues, but not the other, with the human residue enabled tree shrew CD1d to regain the ability to present lipid antigen to human iNKT cells. These results indicate that CD1d/iNKT recognition is species-specific, and that cross-species reactivity may be less common than currently proposed. Also, a naturally occurring CD1d mutation(s) that confers inability to stimulate iNKT cell function may have implications for future studies on CD1d/iNKT-associated diseases. |
| 关键词 | Antigen Presentation Cd1d Antigen t Lymphocytes Tupaiidae |
| 学科领域 | Immunology |
| 资助者 | This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. ; This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team. |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/4433 |
| 专题 | 分子免疫生物学 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | demin.li@imm.ox.ac.uk |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Insti- tute of Zoology, Chinese Academy of Science, Kunming, Yunnan Province, China 2.Graduate University of Chinese Academy of Sciences, Beijing, China 3.MRC Human Immunology Unit, Weatherall Institute of Molecular Medi- cine, John Radcliffe Hospital, Oxford Univer- sity, Oxford, UK 4.Department of Immunology, Duke University Medical Center, Durham, NC, USA |
| 推荐引用方式 GB/T 7714 | Zhang P,Li DM*,Stewart-Jones G,et al. A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells[J]. IMMUNOLOGY,2009,128(4):500-510. |
| APA | Zhang P.,Li DM*.,Stewart-Jones G.,Shao XL.,Zhang YX.,...&demin.li@imm.ox.ac.uk.(2009).A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells.IMMUNOLOGY,128(4),500-510. |
| MLA | Zhang P,et al."A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells".IMMUNOLOGY 128.4(2009):500-510. |
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| 20091284500.pdf（769KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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