Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein

	Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein
其他题名	具有抗HIV-1活性的新核糖体失活蛋白-栝楼蛋白
	Zheng YT 1; Ben KL*1; Jin SW 2; kben@mail.kiz.ac.cn
	2000
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	0253-9756
卷号	21 期号:2 页码:179-182
合作性质	其它
摘要	AIM: To determine whether trichobitacin, a novel ribosome-inactivating protein purified from the root tubers of Trichosanthes kirilowii, possesses the anti-HIV activity. METHODS: The inhibition of syncytial cell formation induced by human immunodeficiency virus type 1 (HIV-1) was determined under microscope, reduction of HIV-1 p24 antigen expression level was measured by ELISA, and decrease in numbers of HIV-1 antigen positive cells in acutely and chronically infected cultures were detected by indirect immunofluorescence assay. RESULTS: Trichobitacin was found to greatly suppress syncytial cell formation induced by HIV-1 and to markedly reduce both expression of HIV-1 p24 antigen and the number of HIV antigen positive cells in acutely but not chronically HIV-1 infected culture. The median inhibitory concentration (IC50) in inhibition of syncytial cell formation and HIV antigen positive cells were 5 micrograms.L-1 (95% confidence limits: 1.3-20 micrograms.L-1) and 0.09 mg.L-1 (95% confidence limits: 0.011-0.755 mg.L-1), respectively. CONCLUSION: Trichobitacin is a novel ribosome-inactivating protein with anti-HIV-1 activity.
其他摘要	目的:研究新核糖体失活蛋白-栝楼蛋白是否具有抗HIV-1活性。方法:合胞体抑制实验以光镜检查,以ELISA测定HIV-1 p24抗原表达水平,用间接免疫荧光技术测定HIV抗原阳性细胞的百分率。结果:栝楼蛋白对HIV-1诱导C8166细胞形成合胞体有显著的抑制作用。栝楼蛋白显著地抑制了HIV-1急性感染T细胞中p24核心抗原的表达水平和减少了HIV抗原阳性细胞的百分率。栝楼蛋白抑制合胞体形成和HIV抗原阳性细胞的IC_(50)分别是5μg·L~(-1)和0.09mg·L~(-1)。栝楼蛋白不影响慢性感染T细胞中HIV-1 p24抗原表达水平。结论:栝楼蛋白是具有抗HIV-1活性的新的核糖体失活蛋白。
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4517
专题	科研部门_分子免疫药理学（郑永唐）
通讯作者	kben@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Zheng YT,Ben KL*,Jin SW,et al. Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein[J]. ACTA PHARMACOLOGICA SINICA,2000,21(2):179-182.
APA	Zheng YT,Ben KL*,Jin SW,&kben@mail.kiz.ac.cn.(2000).Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein.ACTA PHARMACOLOGICA SINICA,21(2),179-182.
MLA	Zheng YT,et al."Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein".ACTA PHARMACOLOGICA SINICA 21.2(2000):179-182.

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