| Relationship between trichosanthin cytotoxicity and its intracellular concentration | |
| Chan WL1; Zheng YT2; Huang H1; Tam SC*1; mtam@cuhk.edu.hk | |
| 2002 | |
| 发表期刊 | TOXICOLOGY
 |
| ISSN | 0300-483X |
| 卷号 | 177期号:2-3页码:245-251 |
| 合作性质 | 其它 |
| 摘要 | Trichosanthin (TCS) is a type I ribosome-inactivating protein with board spectrum of biological activity. Toxicity of this compound differs in different cell lines and this study examined the cause of such difference. It is generally believed that TCS toxicity is mediated through intracellular ribosome inactivation. Therefore, TCS toxicity should be determined by the amount inside cells rather than outside. Three different cell types IC21, JAR and Vero cell lines were chosen with high, medium and low sensitivity to TCS. Intracellular concentrations of fluorescein isothiocyanate labeled TCS were determined by laser scanning confocal microscopy. A good relationship was demonstrated between intracellular TCS concentration and toxicity. Highest intracellular concentration was found in IC21, followed by JAR, and lowest in Vero cells. When the intracellular TCS concentrations in these cells were reduced by using a competitive inhibitor to block cell entry, cytotoxicity was not observed. In conclusion, there is strong evidence to indicate that cytotoxicity of TCS is dependent on its intracellular concentration. Variation of cytotoxicity in different cells may be related to the mechanisms affecting its internalization. |
| 关键词 | Ldl Receptor-related Protein/alpha(2)-macroglobulin Receptor Ribosome-inactivating Protein Trichosanthin Endocytosis Cytotoxicity |
| 资助者 | This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. ; This work was supported by grants 4243/98M from Research Grants Council of Hong Kong SAR. |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/4525 |
| 专题 | 科研部门_分子免疫药理学（郑永唐） |
| 通讯作者 | mtam@cuhk.edu.hk |
| 作者单位 | 1.Department of Physiology, The Chinese Uni?ersity of Hong Kong, Shatin, Hong Kong 2.Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223 , China |
| 推荐引用方式 GB/T 7714 | Chan WL,Zheng YT,Huang H,et al. Relationship between trichosanthin cytotoxicity and its intracellular concentration[J]. TOXICOLOGY,2002,177(2-3):245-251. |
| APA | Chan WL,Zheng YT,Huang H,Tam SC*,&mtam@cuhk.edu.hk.(2002).Relationship between trichosanthin cytotoxicity and its intracellular concentration.TOXICOLOGY,177(2-3),245-251. |
| MLA | Chan WL,et al."Relationship between trichosanthin cytotoxicity and its intracellular concentration".TOXICOLOGY 177.2-3(2002):245-251. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2002177245.pdf（161KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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