The anti-HIV-1 effect of scutellarin

	The anti-HIV-1 effect of scutellarin
	Zhang GH 1,4; Wang Q 1,4; Chen JJ 2; Zhang XM 2; Tam SC 3; Zheng YT*1; zhengyt@post.kiz.ac.cn
	2005
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	334 期号:3 页码:812-816
合作性质	其它
摘要	Scutellarin was purified from the plant Erigeron breviscapus (Vant.) Hand.-Mazz. The activity against 3 strains of human immunodeficiency virus (HIV) was determined in vitro in this study. These were laboratory-derived virus (HIV-1IIIB), drug-resistant virus (HIV-1(74V)), and low-passage clinical isolated virus (HIV-1(KM018)). From syncytia inhibition study, the EC50 of scutellarin against HIV-1IIIB direct infection in C8166 cells was 26 microM with a therapeutic index of 36. When the mode of infection changed from acute infection to cell-to-cell infection, this compound became even more potent and the EC50 reduced to 15 microM. This suggested that cell fusion might be affected by this compound. By comparing the inhibitory effects on p24 antigen, scutellarin was also found to be active against HIV-1(74V) (EC50 253 microM) and HIV-1KM018 (EC50 136 microM) infection with significant difference in potency. The mechanism of its action was also explored in this study. At a concentration of 433 microM, scutellarin inhibited 48% of the cell free recombinant HIV-1 RT activity. It also caused 82% inhibition of HIV-1 particle attachment and 45% inhibition of fusion at the concentrations of 54 microM. In summary, scutellarin was found to inhibit several strains of HIV-1 replication with different potencies. It appeared to inhibit HIV-1 RT activity, HIV-1 particle attachment and cell fusion. These are essential activities for viral transmission and replication.
关键词	Reverse Transcriptase Hiv Entry Scutellarin Hiv-1 Anti-hiv Agent
资助者	The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142).
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收录类别	SCI
语种	英语
资助者	The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142). ; The work was supported by grants to Dr. Zheng from the Natural Science Foundation of China (30471605), the Natural Science Foundation of Yunnan (2002C0066M; 2003C0001R), Key Scientific and Tech- nological projects of of China (2004BA719A14) and Yunnan province (2004NG12), CAS Knowledge Inno- vation Projects (KSCX2-SW-216; KSCX1-SW-11), and National 863 Program (2003AA219142).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4539
专题	科研部门_分子免疫药理学（郑永唐）
通讯作者	zhengyt@post.kiz.ac.cn
作者单位	1.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650204, China 3.Department of Physiology, The Chinese University of Hong Kong, Hong Kong SAR, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Zhang GH,Wang Q,Chen JJ,et al. The anti-HIV-1 effect of scutellarin[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,334(3):812-816.
APA	Zhang GH.,Wang Q.,Chen JJ.,Zhang XM.,Tam SC.,...&zhengyt@post.kiz.ac.cn.(2005).The anti-HIV-1 effect of scutellarin.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,334(3),812-816.
MLA	Zhang GH,et al."The anti-HIV-1 effect of scutellarin".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 334.3(2005):812-816.

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