| Enhanced apoptotic action of trichosanthin in HIV-1 infected cells | |
| Wang YY1,3; Ouyang DY1,3; Huang H2; Chan H2; Tam SC2; Zheng YT*1; zhengyt@mail.kiz.ac.cn | |
| 2005 | |
| 发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| ISSN | 0006-291X |
| 卷号 | 331期号:4页码:1075-1080 |
| 摘要 | Trichosanthin (TCS) is a type 1 ribosome-inactivating protein (RIP) effective against HIV-1 replication. The mechanism is not clear. Present results suggested that the antiviral action may be partly mediated through enhanced apoptosis on infected cells. TCS induced apoptosis in normal H9 cells and this action was more potent in those infected with HIV-1. In flow cytometry study, TCS induced larger population of apoptotic H9 cells chronically infected with HIV-1 in a dose-dependent manner. At TCS concentration of 25 microg/ml, 8.4% of normal H9 cells were found to be apoptotic whereas the same concentration induced 24.5% in HIV-1 chronically infected cells. Such difference was not found in the control experiments without TCS treatment. Two other studies supported this action. Cytotoxic study showed that cell viability was always lower in HIV-1 infected cells after TCS treatment, and DNA fragmentation study confirmed more laddering in infected cells. The mechanism of TCS induced apoptosis in normal or infected H9 cells is not clear. Results in this study demonstrated that TCS is more effective in inducing apoptosis in HIV-1 infected cells. This may explain in part the antiviral action of TCS. |
| 关键词 | Trichosanthin Hiv-1 Apoptosis Infected Cells Anti-hiv Activity |
| 资助者 | The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (30471605) and Yunnan Province (2002C0066M; 2003C0022R); CAS Knowledge Innova- tion Projects (KSCX2-SW-216), Key Scientific and Technological projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/4545 |
| 专题 | 科研部门_分子免疫药理学（郑永唐） |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 作者单位 | 1.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Department of Physiology, The Chinese University of Hong Kong, Hong Kong SAR, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Wang YY,Ouyang DY,Huang H,et al. Enhanced apoptotic action of trichosanthin in HIV-1 infected cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,331(4):1075-1080. |
| APA | Wang YY.,Ouyang DY.,Huang H.,Chan H.,Tam SC.,...&zhengyt@mail.kiz.ac.cn.(2005).Enhanced apoptotic action of trichosanthin in HIV-1 infected cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,331(4),1075-1080. |
| MLA | Wang YY,et al."Enhanced apoptotic action of trichosanthin in HIV-1 infected cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 331.4(2005):1075-1080. |
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| 20053311075.pdf（492KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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