Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells

	Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells
	Huang H 1; Chan H 1; Wang YY 2,3; Ouyang DY 2,3; Zheng YT 2; Tam SC*1; mtam@cuhk.edu.hk
	2006
发表期刊	LIFE SCIENCES
ISSN	0024-3205
卷号	79 期号:13 页码:1287-1292
合作性质	其它
摘要	Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) effective against HIV-1 and HSV-1 replication. The mechanism of its antiviral activity is not clear. Many believe that it is related to ribosome inactivation. Some RIPs and viral infection affect the phosphorylation of MAPK and Bcl-2 and these proteins may be the common element linking RIP and viral infection. This study investigated the effect of HSV-1 infection on p38 MAPK and Bcl-2 as well as possible interference by TCS. Results showed that HSV-1 infection induced an elevation of phosphorylated p38 and Bcl-2 in Vero cells, which could be partially blocked by TCS. At the same time, both viral replication and host cells viability were lowered. Viral replication, Vero cell viability, p38 MAPK and Bcl-2 were further reduced with the addition of a p38 MAPK inhibitor (SB203580). This suggested that TCS may interfere with MAPK and Bcl-2 signals generated by infection leading to inhibition of viral replication. In summary, our results demonstrated that HSV-1 infection in Vero cells induced an elevation of p38 MAPK and Bcl-2. TCS suppressed this rise and reduced viral replication. The MAPK family may play a role in the antiviral mechanism of TCS.
关键词	Trichosanthin Hsv-1 Mapk Bcl-2 Antiviral Activity
资助者	The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142).
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收录类别	SCI
资助者	The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142). ; The work was supported by grants from the Natural Science Foundation of China (39970851) and Yunnan Province (2002C0066M); CAS Knowledge Innovation Projects (KSCX2-SW-216), Key Scientific and Technological Projects of China (2004BA719A14) and Yunnan Province (2004NG12), and 863 Program (2003AA219142).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4557
专题	科研部门_分子免疫药理学（郑永唐）
通讯作者	mtam@cuhk.edu.hk
作者单位	1.Department of Physiology, The Chinese University of Hong Kong, Hong Kong SAR, China 2.Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Huang H,Chan H,Wang YY,et al. Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells[J]. LIFE SCIENCES,2006,79(13):1287-1292.
APA	Huang H.,Chan H.,Wang YY.,Ouyang DY.,Zheng YT.,...&mtam@cuhk.edu.hk.(2006).Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells.LIFE SCIENCES,79(13),1287-1292.
MLA	Huang H,et al."Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells".LIFE SCIENCES 79.13(2006):1287-1292.

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