Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein

	Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein
	Liu GJ 1; Wang JP 1; Xiao JC 1,2; Zhao ZW 2; Zheng YT*1; zhengyt@mail.kiz.ac.cn
	2007
发表期刊	CELLULAR & MOLECULAR IMMUNOLOGY
ISSN	1672-7681
卷号	4 期号:3 页码:203-208
摘要	HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies (mAbs) p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1IIIB, HIV-1Ada-M, HIV-174V mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research.
关键词	Monoclonal Antibody Hiv-1 P24 Epitope Elisa
资助者	The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15).
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收录类别	SCI
语种	英语
资助者	The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15). ; The work was supported in part by grants from the National Natural Science Foundation of China (39500137, 30471605, 30671960); the Natural Science Foundation of Yunnan (95C0099Q); Key Technological R&D Program of China (2004BA719A14) and Yunnan (2004NG12); CAS Projects (KSZ85-0108, STZ-01-17; KSCX2-SW-216; KSCX1-SW-11, KSCX1-YW-R-15).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4583
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhengyt@mail.kiz.ac.cn
作者单位	1.Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Key Laboratory of Conservation and Utilization for Bio-resources, Yunnan University, Kunming, Yunnan 650091, China
推荐引用方式 GB/T 7714	Liu GJ,Wang JP,Xiao JC,et al. Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2007,4(3):203-208.
APA	Liu GJ,Wang JP,Xiao JC,Zhao ZW,Zheng YT*,&zhengyt@mail.kiz.ac.cn.(2007).Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein.CELLULAR & MOLECULAR IMMUNOLOGY,4(3),203-208.
MLA	Liu GJ,et al."Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein".CELLULAR & MOLECULAR IMMUNOLOGY 4.3(2007):203-208.

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