Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis

	Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis
	Wang RR 1,4; Gu Q 2,4; Wang YH 1,4; Zhang XM 2,3; Yang LM 1,3; Zhou J 2,3; Chen JJ 2,3; Zheng YT*1,3; zhengyt@mail.kiz.ac.cn
	2008
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	117 期号:2 页码:249-256
摘要	Aim of the study: Previously, we reported that the petroleum ether fraction, RC-1, and EtOAc fraction, RC-2, of the medicinal plant Rhus chinensis showed potent anti-HIV-1 activities. To address anti-HIV-1 constituents of RC-1 and RC-2, 17 compounds were isolated. Anti-HIV-1 activities and possible action mechanisms of these compounds were investigated. Methods: The syncytial formation induced by HIV-1 was determined under the inverted microscope, cellular toxicity and protection assay were assessed by MTT method, reduction of p24 antigen expression level and RT activity were measured by ELISA, and inhibition of recombinant HIV-1 PR was monitored by the fluorescent signal. Results: The compounds 1 and 13 inhibited HIV-1-induced syncytium formation potently with TI value of 42.31 and 19.07, respectively. Compounds 4, 5, 6, 9 and 10 were less potent with TI value of 8.94, 8.22, 4.14, 5.11 and 5.34, respectively. Compound 1, a benzofuranone-type compound, previously reported as a novel anti-HIV-1 agent, might target late-steps of HIV-1 life cycle. Compound 13 inhibited HIV-1 replication with EC50 of 7.16 mu g/ml and might target at/before integration step. Conclusion: These compounds might contribute to anti-HIV-1 activities extracts of the medicinal plant Rhus chinensis.
关键词	Rhus Chinensis Hiv-1 Benzofuranone Anti-hiv Agents Botanicals
资助者	The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12).
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收录类别	SCI
语种	英语
资助者	The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12). ; The work was supported by grants from the Chinese Academy of Sci- ences (KSCX1-YW-R-15, KSCX1-YW-R-24), National 973 project of China (2006CB504208, 2006CB504302), the Nat- ural Science Foundation of China (30471605, 30671960), and Key Scientific and Technological projects of Yunnan (2004NG12).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4635
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhengyt@mail.kiz.ac.cn
作者单位	1.Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650204, China 3.The Joint-Laboratory of Antiviral Natural Medicines, Kunming Branch, Chinese Academy of Sciences, Kunming, Yunnan, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Wang RR,Gu Q,Wang YH,et al. Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis[J]. JOURNAL OF ETHNOPHARMACOLOGY,2008,117(2):249-256.
APA	Wang RR.,Gu Q.,Wang YH.,Zhang XM.,Yang LM.,...&zhengyt@mail.kiz.ac.cn.(2008).Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis.JOURNAL OF ETHNOPHARMACOLOGY,117(2),249-256.
MLA	Wang RR,et al."Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis".JOURNAL OF ETHNOPHARMACOLOGY 117.2(2008):249-256.

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