Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques

	Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques
	Xia HJ 1,2; Liu HL 1,2; Zhang GH 1; Zheng YT*1; zhengyt@mail.kiz.ac.cn
	2009
发表期刊	CELLULAR & MOLECULAR IMMUNOLOGY
ISSN	1672-7681
卷号	6 期号:3 页码:159-165
摘要	Dendritic cells (DCs) play a pivotal role in linking the innate immunity and acquired immunity in responses to pathogen. Non-human primates such as Chinese Rhesus Macaque (CRM) are the favorable models for preclinical study of potential therapeutic drugs, vaccines and mechanisms of human diseases. However, the phenotypical characterization of monocyte-derived dendritic cells (MDDCs) from CRM has not been elucidated. Monocytes from CRM were cultured with GM-CSF and IL-4 in RPMI-1640. Six days later, these cells were differentiated with typical dendritical morphology. CD11c and DC-SIGN were highly expressed. The immature MDDCs expressed the low levels of CD25, CD80, CD83, moderate CD40, CD86, and high MHC. After stimulation, the mature MDDCs increased expression of mature molecules CD25 and CD83, co-stimulatory molecules such as CD80, CD86 and CD40, and kept a high level of MHC. The capacity of endocytosis decreased with maturation. The mature MDDCs have strong ability of inducing allogeneic T cell proliferation and producing IL-12. In conclusion, we have characterized the phenotype and ultimate function of MDDCs from CRM for the first time. Cellular & Molecular Immunology. 2009;6(3):159-165.
关键词	Pentylenetetrazol Brief Seizures Morris Water Maze Contextual Fear Conditioning Acquisition Consolidation
资助者	This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript.
收录类别	SCI
语种	英语
资助者	This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript. ; This work was supported in part by grants from Scientific and Technological projects of China (2008ZX10001-002, 2008ZX10001-013, 2008ZX10005-005) and Yunnan (2006PT08), 973 Program (2006CB504208), the NSFC (30471605, 30671960, U0832601), and the CAS (KSCX1-YW-R-15) to Dr. YT Zheng, and “Western Light” Projects to Dr. GH Zhang. We also acknowledge Dr. Michael SC Tam for critical and constructive comments on the manuscript.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4665
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhengyt@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Xia HJ,Liu HL,Zhang GH,et al. Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques[J]. CELLULAR & MOLECULAR IMMUNOLOGY,2009,6(3):159-165.
APA	Xia HJ,Liu HL,Zhang GH,Zheng YT*,&zhengyt@mail.kiz.ac.cn.(2009).Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques.CELLULAR & MOLECULAR IMMUNOLOGY,6(3),159-165.
MLA	Xia HJ,et al."Phenotype and Function of Monocyte-Derived Dendritic Cells from Chinese Rhesus Macaques".CELLULAR & MOLECULAR IMMUNOLOGY 6.3(2009):159-165.

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