A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand

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	A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand
	Li D 1,2; Dong Y 1,2; Jiang Y 1,2; Jiang HF 1; Cai J 1,2; Wang W*1; wwang@mail.kiz.ac.cn
	2010
发表期刊	CELL RESEARCH
ISSN	1001-0602
卷号	20 期号:4 页码:408-420
合作性质	其它
摘要	Recent transcription profiling studies have revealed an unexpectedly large proportion of antisense transcripts in eukaryotic genomes. These antisense genes seem to regulate gene expression by interacting with sense genes. Previous studies have focused on the non-coding antisense genes, but the possible regulatory role of the antisense protein is poorly understood. In this study, we found that a protein encoded by the antisense gene ADF1 acts as a transcription suppressor, regulating the expression of sense gene MDF1 in Saccharomyces cerevisiae. Based on the evolutionary, genetic, cytological and biochemical evidence, we show that the protein-coding sense gene MDF1 most likely originated de novo from a previously non-coding sequence and can significantly suppress the mating efficiency of baker's yeast in rich medium by binding MAT alpha 2 and thus promote vegetative growth. These results shed new light on several important issues, including a new sense-antisense interaction mechanism, the de novo origination of a functional gene, and the regulation of yeast mating pathway.
关键词	De Novo Sense-antisense Interaction Mating Pathway Saccharomyces Cerevisiae
资助者	This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment.
收录类别	SCI
语种	英语
资助项目	This work was supported by a CAS key grant (KSCX2-YW-N-060), two NSFC key grants (No. 30930056 and 30623007), a 973 Program (No. 2007CB815703-5), a CAS-Max Planck Society Fellowship, and the 100 Talent Program of CAS to W.W.
资助者	This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment. ; This work was funded by grants awarded to S. Zhang under the Key Construction Program of the National "985" Project and "211" Project. J. Flanders was supported by a Department for Innovation, Universities and Skills Fellowship (UK). We thank Mr. Junpeng Zhang, Dr. Libiao Zhang, Dr. Guangjian Zhu, Dr. Jinshuo Zhang, Dr. Xiuguang Mao and Dr. Li Wei for their assistance in the field. We would also like to thank Dr. Xiangdong Tang and his wife for technological support. Finally, we thank Dr. Byron Shen the DSI Company equipment.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4863
专题	基因起源组遗传资源与进化国家重点实验室
通讯作者	wwang@mail.kiz.ac.cn
作者单位	1.CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zool- ogy, Chinese Academy of Sciences (CAS), Kunming, Yunnan 650223, China 2.Graduate School of Chinese Academy Sciences, Bei- jing 100039, China
推荐引用方式 GB/T 7714	Li D,Dong Y,Jiang Y,et al. A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand[J]. CELL RESEARCH,2010,20(4):408-420.
APA	Li D.,Dong Y.,Jiang Y.,Jiang HF.,Cai J.,...&wwang@mail.kiz.ac.cn.(2010).A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand.CELL RESEARCH,20(4),408-420.
MLA	Li D,et al."A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand".CELL RESEARCH 20.4(2010):408-420.

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