Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks

	Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks
	He XL 1,3; Qian WF 1; Wang Z 1; Li Y 1,2; Zhang JZ[*]1; jianzhi@umich.edu
	2010
发表期刊	NATURE GENETICS
ISSN	1061-4036
卷号	42 期号:3 页码:272-U120
合作性质	其它
摘要	Epistasis refers to the interaction between genes. Although high-throughput epistasis data from model organisms are being generated and used to construct genetic networks(1-3), the extent to which genetic epistasis reflects biologically meaningful interactions remains unclear(4-6). We have addressed this question through in silico mapping of positive and negative epistatic interactions amongst biochemical reactions within the metabolic networks of Escherichia coli and Saccharomyces cerevisiae using flux balance analysis. We found that negative epistasis occurs mainly between nonessential reactions with overlapping functions, whereas positive epistasis usually involves essential reactions, is highly abundant and, unexpectedly, often occurs between reactions without overlapping functions. We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs.
资助者	This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q).
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收录类别	SCI
语种	英语
资助者	This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q). ; This project was supported financially by file NSFC (No. 20802082) and 30830115) and funds from the Chinese Academy of Sciences ("Xibuzhiguang" to W.-L.X and No. KSCX1-YW-R-24), the Major State Basic Research Development program of China (Nos. 2009CB522303 and 2009CB940900), the Young Academic and Technical Leader Rising Foundation of Yunnan province (2006PY0147), and the Natural Science Foundation of Yunnan Province (2005XY04 and 2006B0042Q).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4909
专题	科研部门_分子进化与基因组多样性（张亚平）
通讯作者	jianzhi@umich.edu
作者单位	1.Department of Ecology and Evolutionary Biology, Ann Arbor, Michigan, USA 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 3.Present address: State Key Laboratory of Bio-control, College of Life Sciences, Sun Yat-Sen University, Guangzhou, China
推荐引用方式 GB/T 7714	He XL,Qian WF,Wang Z,et al. Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks[J]. NATURE GENETICS,2010,42(3):272-U120.
APA	He XL,Qian WF,Wang Z,Li Y,Zhang JZ[*],&jianzhi@umich.edu.(2010).Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks.NATURE GENETICS,42(3),272-U120.
MLA	He XL,et al."Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks".NATURE GENETICS 42.3(2010):272-U120.

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