A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom

KIZ OpenIR > 其他

	A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom
	Li YS 1; Liu KF 1; Wang QC*1; Ran YL 2; Tu GC 2
	1985
发表期刊	TOXICON
ISSN	0041-0101
卷号	23 期号:6 页码:859-903
合作性质	其它
摘要	Y.-S. Li, K.-F. Liu, Q.-C. Wang, Y.-L. Rpan and G.-C. Tu. A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom. Toxicon23, 895–903, 1985. — By means of CM-Sephadex C-50 column chromatography and gel filtration on Sephadex G — 75, a potent platelet function inhibitor was purified from Vipera russelli siamensis venom. It appeared as a single protein band on polyacrylamide gel electrophoresis in the presence or absence of SDS, and consists of 123 amino acid residues. Its NH3-terminal residue is serine. It showed the following characteristics: molecular weight, 13,800; isoelectric point, 10.4; ld(In50), 0.5 ± 0.12 mg/kg (i.v.). The platelet inhibitor exhibited phospholipase A2 activity with a specific activity of 35 μmoles/min/mg. From 2 g of the venom, 70 mg of the purified inhibitor was obtained. Inhibition of human platelet aggregation induced by ADP or adrenaline was dose-dependent, with id(In50) of 1.14μg/ml or 0.37 μg/ml, respectively. The platelet aggregation induced by thrombin or collagen was also inhibited and the inhibitory activity on platelet aggregation was heat stable (at 100°C, 20 min) in an acidic medium (pH 5.8), while its phospholipase A2 activity was relatively heat labile under the same condition. The release of 3H-serotonin in platelets stimulated by ADP was also inhibited and this was positively correlated with inhibition of platelet aegregation induced by ADP (r = 0.998, P < 0.002).
部门归属	其他
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/4991
专题	其他
作者单位	1.Department of Pharmacology, Fujian Medical College, Fuzhou, People's Republic of China 2.Kunming Institute of Zoology, Academia Sinica, Kunming, People's Republic of China
推荐引用方式 GB/T 7714	Li YS,Liu KF,Wang QC*,et al. A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom[J]. TOXICON,1985,23(6):859-903.
APA	Li YS,Liu KF,Wang QC*,Ran YL,&Tu GC.(1985).A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom.TOXICON,23(6),859-903.
MLA	Li YS,et al."A platelet function inhibitor purified from Vipera russelli siamensis (Smith) snake venom".TOXICON 23.6(1985):859-903.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2016120101.pdf（457KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文