KMS KUNMING INSTITUTE OF ZOOLOGY.CAS
| Vasectomy-induced autoimmunity: monoclonal antibodies affect sperm function and in vitro fertilization | |
| Ben K1; Hamilton MS2; Alexander NJ*3 | |
| 1988 | |
| 发表期刊 | JOURNAL OF REPRODUCTIVE IMMUNOLOGY
 |
| ISSN | 0165-0378 |
| 卷号 | 13期号:1页码:73-84 |
| 摘要 | A panel of sperm-reacted monoclonal auto-antibodies developed from spleen cells of vasectomized mice (BDF1) were characterized. Immuogenic antigens were mainly located on the acrosome, midpiece and principal piece. All of the monoclonals were IgM; three demonstrated a multispecific reaction with testis and/or epididymis antigen extracts by immunoblotting. Immunobead studies indicated that most of the antibodies were to surface molecules, a finding supported by the observation that five of the seven antibodies caused complement-mediated immobilization (although not sperm agglutination) of mouse sperm. In vitro fertilization was significantly impaired when antibodies (Vx5, 8 and 10) were added to the sperm prior to exposure to the eggs. Furthermore, passive immunization with Vx5 antibody reduced in vivo fertilization. Our findings indicate that vasectomy-generated antibodies can reduce sperm function in vitro and in vivo. |
| 部门归属 | 其他 |
| 关键词 | Vasectomy Monoclonal Antibodies Mouse In Vitro Fertilization In Vivo Fertilization Sperm Immobilization |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5025 |
| 专题 | 其他 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan China 2.Department of Biological Structure, University of Washington School of Medicine, Seattle, Washington U.S.A 3.Section of Reproductive Immunology, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Lewis Hall, Virginia U.S.A. |
| 推荐引用方式 GB/T 7714 | Ben K,Hamilton MS,Alexander NJ*. Vasectomy-induced autoimmunity: monoclonal antibodies affect sperm function and in vitro fertilization[J]. JOURNAL OF REPRODUCTIVE IMMUNOLOGY,1988,13(1):73-84. |
| APA | Ben K,Hamilton MS,&Alexander NJ*.(1988).Vasectomy-induced autoimmunity: monoclonal antibodies affect sperm function and in vitro fertilization.JOURNAL OF REPRODUCTIVE IMMUNOLOGY,13(1),73-84. |
| MLA | Ben K,et al."Vasectomy-induced autoimmunity: monoclonal antibodies affect sperm function and in vitro fertilization".JOURNAL OF REPRODUCTIVE IMMUNOLOGY 13.1(1988):73-84. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 19881373.pdf（624KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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