Secretory monoclonal IgA antibody to human sperm produced by gastrointestinal immunization inhibits human sperm activity and mouse in vitro fertilization

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	Secretory monoclonal IgA antibody to human sperm produced by gastrointestinal immunization inhibits human sperm activity and mouse in vitro fertilization
	Cao XM; Ben KL*; Ma L; Wang YM; Chen YL; Zhou HM
	1993
发表期刊	JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN	0165-0378
卷号	24 期号:1 页码:13-28
合作性质	其它
摘要	BALB/c mice were immunized intragastrically with human sperm. Cells from the Peyer's patches and spleens of the immunized mice were for the preparation of hybridomas secreting antisperm monoclonal IgA (mcIgA). The specific ratio of IgA-secreting cells in Peyer's patches was much higher than that in spleen. The binding site on human sperm of 9 of 19 mcIgA was in the post-acrosomal region using an immunofluorescent assay. Two of eight selected mcIgA caused strong human sperm agglutination and three of them produced significant inhibition of mouse in vitro fertilization. No mcIgA tested caused obvious human sperm immobilization or inhibited mouse in vivo fertilization. In vitro assembly of selected mcIgA in ascites with mouse secretory component (SC) caused no significant changes in effects on sperm function and in vitro fertilization. By use of Western blotting, dimer or higher polymers were demonstrated in all selected mcIgAs and corresponding protein antigens in 6 of 8 selected mcIgAs. These results suggest that human sperm function may be inhibited and fertilization rate reduced by specific secretory IgA to human sperm and that secretory immunity to protein antigens of human sperm could be induced by intragastrointestinal immunization.
部门归属	其他
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5079
专题	其他
作者单位	Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223 People's Republic of China
推荐引用方式 GB/T 7714	Cao XM,Ben KL*,Ma L,et al. Secretory monoclonal IgA antibody to human sperm produced by gastrointestinal immunization inhibits human sperm activity and mouse in vitro fertilization[J]. JOURNAL OF REPRODUCTIVE IMMUNOLOGY,1993,24(1):13-28.
APA	Cao XM,Ben KL*,Ma L,Wang YM,Chen YL,&Zhou HM.(1993).Secretory monoclonal IgA antibody to human sperm produced by gastrointestinal immunization inhibits human sperm activity and mouse in vitro fertilization.JOURNAL OF REPRODUCTIVE IMMUNOLOGY,24(1),13-28.
MLA	Cao XM,et al."Secretory monoclonal IgA antibody to human sperm produced by gastrointestinal immunization inhibits human sperm activity and mouse in vitro fertilization".JOURNAL OF REPRODUCTIVE IMMUNOLOGY 24.1(1993):13-28.

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