Hormonal regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured monkey Sertoli cells

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	Hormonal regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured monkey Sertoli cells
	Liu YX*1; Liu K 1,3; Zhou HM 1; Du Q 1,4; Hu ZY 1; Zou RJ 2
	1995
发表期刊	Molecular Human Reproduction
ISSN	0268-1161
卷号	10 期号:3 页码:719-727
摘要	Sertoli cells play a central role in the control and maintenance of spermatogenesis. Isolated Sertoli cells of mouse and rat testes have been shown to secrete plasminogen activator (PA) and a plasminogen activator inhibitor type-1 (PAI-1) in culture. In this study, we have investigated the hormonal regulation of PA and PAI-1 activities in cultured monkey Sertoli cells. Sertoli cells (5x10(5) cells/well) isolated from infant rhesus monkey testes were preincubated at 35 degrees C for 16 h in 24-well plates precoated with poly(D-lysine) (5 mu g/cm(2)) in 0.5 mi McCoy's 5a medium containing 5% of fetal calf serum and further incubated for 48 h in 0.5 mi serum-free medium with or without various hormones or other compounds, PA as well as PAI-1 activities in the conditioned media were assayed by fibrin overlay and reverse fibrin autography techniques respectively. The Sertoli cells in vitro secreted only tissue-type PA (tPA), no detectable amount of urokinase-type PA (uPA) could be observed, Monkey Sertoli cells were also capable of secreting PAI-1, Immunocytochemical studies indicated that both tPA and PAI-1 positive staining localized in the Sertoli cells, spermatids and residual bodies of the seminiferous epithelium; Northern blot analysis further confirmed the presence of both tPA and PAI-1 mRNA in monkey Sertoli cells. Addition of follicle-stimulating hormone (FSH) or cyclic adenosine monophosphate (cAMP) derivatives or cAMP-generating agents and gonadotrophin-releasing hormone (GnRH) agonist or phorbol ester (PMA) to the cell culture significantly increased tPA activity. PAI-1 activity in the culture was also enhanced by these reagents except 8-bromo-dibutyryl-cAMP, forskolin and 3-isobutyl-1-methylxanthin (MIX) which greatly stimulated tPA activity, whereas decreased PAI-1 activity, implying that neutralization of PAI-1 activity by tile high level of tPA in the conditioned media may occur. These data suggest that increased intracellular signals which activate protein kinase A (PKA), or protein kinase C (PKC) can modulate Sertoli cell tPA and PAI-1 activities, The concomitant induction of PA and PAI-1 by the same reagents in the Sertoli cells may reflect a finely tuned regulatory mechanism in which PAI-1 could limit the excession of the proteolysis.
资助者	This research was supported by the National Natural Science Foundation of China and the State Family Planning Commission. ; This research was supported by the National Natural Science Foundation of China and the State Family Planning Commission.
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收录类别	SCI
语种	英语
资助者	This research was supported by the National Natural Science Foundation of China and the State Family Planning Commission. ; This research was supported by the National Natural Science Foundation of China and the State Family Planning Commission.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5109
专题	其他
作者单位	1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences 2.Primate Research Center, Kunming Institute of Zoology, Kunming 650223, China 3.Present address: Department of Medical Biochemistry- Umea University, S-90187 Umea, Sweden 4.Present address: Department of Pathology, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
推荐引用方式 GB/T 7714	Liu YX*,Liu K,Zhou HM,et al. Hormonal regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured monkey Sertoli cells[J]. Molecular Human Reproduction,1995,10(3):719-727.
APA	Liu YX*,Liu K,Zhou HM,Du Q,Hu ZY,&Zou RJ.(1995).Hormonal regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured monkey Sertoli cells.Molecular Human Reproduction,10(3),719-727.
MLA	Liu YX*,et al."Hormonal regulation of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 in cultured monkey Sertoli cells".Molecular Human Reproduction 10.3(1995):719-727.

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