Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy

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	Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy
	Cao XM; Ben KL*; Wang YQ; Wang YM; immkiz@public.km.yn.cn
	1997
发表期刊	ANIMAL REPRODUCTION SCIENCE
ISSN	0378-4320
卷号	49 期号:2-3 页码:225-234
摘要	A number of acrosome reaction (AR) initiators have been found to be effective in inducing AR of human, laboratory and domestic animal sperm. Using an improved simple fluorescence microscopy, effects of gamma-aminobutyric acid (GABA), progesterone and ionophore A23187 on sperm AR of tree shrew, a useful animal model in biomedical research, have been investigated. Spontaneous AR in 4.92-7.53% of viable sperm was observed. Complete AR in 10.31-18.25% of viable tree shrew sperm was obviously induced by 5 microM and 10 microM calcium ionophore A23187, 1 mM GABA, and 5 microM progesterone, and there were no significant differences between their abilities to initiate complete AR. No significant differences of AR percentages between 1- and 2-h treatments with A23187, progesterone and/or GABA were observed. These results suggested that the responses of tree shrew sperm to these AR initiators are similar to that of human and other mammalian sperm.
关键词	Acrosome Reaction Initiator Tritc-psa Hoechst 33258 Ionophore Gaba Spontaneous Acrosome Reaction Tree Shrew Sperm
资助者	This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037). ; This work was supported by the Special Programme of Research, Development and Research Training in Human Reproduction of the World Health Organization (WHO/HRP 90037).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5121
专题	其他
通讯作者	immkiz@public.km.yn.cn
作者单位	Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Peoples Republic of China
推荐引用方式 GB/T 7714	Cao XM,Ben KL*,Wang YQ,et al. Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy[J]. ANIMAL REPRODUCTION SCIENCE,1997,49(2-3):225-234.
APA	Cao XM,Ben KL*,Wang YQ,Wang YM,&immkiz@public.km.yn.cn.(1997).Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy.ANIMAL REPRODUCTION SCIENCE,49(2-3),225-234.
MLA	Cao XM,et al."Effects of gamma-aminobutyric acid, progesterone and ionophore A23187 on acrosome reaction of tree shrew sperm in vitro: examination of acrosome reaction with an improved fluorescence microscopy".ANIMAL REPRODUCTION SCIENCE 49.2-3(1997):225-234.

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