Gene mapping of a new coat mutation in mouse

KIZ OpenIR > 其他

	Gene mapping of a new coat mutation in mouse
	Li SR 1; Wang DP 1; Lan H 2; Zhang WY 2; Ge BS 3; Li JQ 1; Wang C 4; Lu YF 5; Shi YY 1; Li RF 1
	1999
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	44 期号:13 页码:1207-1210
摘要	Gene mapping of a mouse coat mutation has been investigated. First, 100 10-bp random primers were used to amplify DNA, but the mutation could not be located by this method because there were no correlation between the amplified products and coat phenotypes. Second, by using Idh1, Car2, Mup1, Pgb1, Hbb, Es10, Es1, Mod1, Gdc1, Ce2, Es3 as genetic markers, linkage test crosses (two-point test) consisting of intercrossing uncovered BALB/c mice (homozygotes) to CBA/N and C57BL/6 mice with normal hair and backcrossing the heterozygotes of the F1 to the uncovered BALB/c mice were made. It was soon evident that the mutation was linked to Es3 on chromosome 11. Furthermore, three-point test was made by using Es3 and D11Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked to Es3 with the percentage recombination of (7.89 +/- 2.19)%, and linked to D11Mit8 with the percentage recombination of (26.38 +/- 3.57)%. The percentage recombination between Es3 and D11Mit8 was (32.90 +/- 3.81)%. The mutation was named Uncovered, with the symbol Uncv. According to the recombinations, the loci order was D11Mit8-26.30 +/- 3.57- Uncv-7.89 +/- 2.19-Es3. From the location on the chromosome, it was concluded that the mutation was a new mutation which affected the skin and hair structure of mouse. The Uncv has entered MGD (Mouse Genome Database).
关键词	Mouse Mutation Gene Mapping
URL	查看原文
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5161
专题	其他
作者单位	1.Inst Jingfeng Med Lab Anim, Beijing 100071, Peoples R China 2.Chinese Acad Sci, Kunming Inst Zool, Kunming 650107, Peoples R China 3.Changchun Univ Agr & Anim Sci, Changchun 130062, Peoples R China 4.Beijing Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China 5.Beijing Inst Biotechnol, Beijing 100071, Peoples R China
推荐引用方式 GB/T 7714	Li SR,Wang DP,Lan H,et al. Gene mapping of a new coat mutation in mouse[J]. CHINESE SCIENCE BULLETIN,1999,44(13):1207-1210.
APA	Li SR.,Wang DP.,Lan H.,Zhang WY.,Ge BS.,...&Li RF.(1999).Gene mapping of a new coat mutation in mouse.CHINESE SCIENCE BULLETIN,44(13),1207-1210.
MLA	Li SR,et al."Gene mapping of a new coat mutation in mouse".CHINESE SCIENCE BULLETIN 44.13(1999):1207-1210.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1999441207.pdf（306KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文