Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones

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	Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones
	Wang XL; Zhao XD; Ben KL*; Cao XM; Wang YQ; Zhou HM; kben@mail.kiz.ac.cn
	2002
发表期刊	IMMUNOLOGY
ISSN	0019-2805
卷号	106 期号:3 页码:343-353
合作性质	其它
摘要	Antigen-specific circulating immunoglobulin-secreting cells (ISC) migrate to various secondary and tertiary lymphoid tissues. To understand the migration of the cells into the genital tract and its regulation by sex hormones, spleen-derived SG2 hybridoma cells secreting immunoglobulin G2b (IgG2b) and Peyer's patch-derived PA4 hybridoma cells secreting polymer IgA were labelled with (3) H-TdR, and intravenously injected into syngeneic mice of both sexes. Using flow cytometry, surface molecular markers of plasma cells, CD38 and CD138, and adhesion molecules, CD49d, CD162, and CD11a were found to be positive in SG2 and PA4 cells, but CD62L, alpha4beta7 and CD44 were not expressed on these cells. The relative distribution indexes (RDIs) of the cells in genital tract and other tissues were measured. The means of RDIs of SG2 and PA4 cells in female genital tissues were 6.5 and 4.5 times as many as the means in male genital tissues, respectively. The treatment of ovariectomized mice with beta-oestradiol significantly increased the RDIs of PA4 cells in cervix and vagina, but decreased the RDIs of SG2 cells in vagina, horn of uterus, uterus and rectum (P <0.05). Progesterone treatment increased the RDIs of PA4 cells in vagina and rectum (P <0.05). The treatment with testosterone significantly increased the RDIs of SG2 and PA4 cells in epididymis and accessory sex glands (P <0.05). These results demonstrate that the female genital tract is the preferable site for the migration of circulating hybridoma cells to the male genital tract, and sex hormones play an important role in regulation of the migration of circulating ISC to genital tracts.
资助者	This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045).
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收录类别	SCI
语种	英语
资助者	This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045). ; This work was supported by National Natural Science Foundation of China (39600016), Applied and Basic Research Fund of Yunnan (96C097Q), and Rockefeller Foundation (88045).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5239
专题	其他科研部门_肿瘤干细胞生物学（赵旭东）
通讯作者	kben@mail.kiz.ac.cn
作者单位	Laboratory for Molecular and Cell Immunology, Kunming Institute of Zoology, The Chinese Academy of Sciences, China
推荐引用方式 GB/T 7714	Wang XL,Zhao XD,Ben KL*,et al. Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones[J]. IMMUNOLOGY,2002,106(3):343-353.
APA	Wang XL.,Zhao XD.,Ben KL*.,Cao XM.,Wang YQ.,...&kben@mail.kiz.ac.cn.(2002).Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones.IMMUNOLOGY,106(3),343-353.
MLA	Wang XL,et al."Circulating immunoglobulin A- and immunoglobulin G-secreting hybridoma cells in peripheral blood preferably migrate to female genital tracts. The role of sex hormones".IMMUNOLOGY 106.3(2002):343-353.

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