Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3

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	Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3
	Cheng Y 1; Meng QX 2; Wang WY 2; Wang JF*1; jfw@sun5.ibp.ac.cn
	2002
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA
ISSN	0167-4838
卷号	1594 期号:2 页码:353-363
合作性质	其它
摘要	Three homologous short-chain neurotoxins, named NT1, NT2 and NT3, were purified from the venom of Naja kaouthia. NT1 has an identical amino acid sequence to cobrotoxin from Naja naja atra [Biochemistry 32 (1993) 2131]. NT3 shares the same sequence with cobrotoxin b [J. Biochem. (Tokyo) 122 (1997) 1252], whereas NT2 is a novel 6 1 -residue neurotoxin. Tests of their physiological functions indicate that NT1 shows a greater inhibition of muscle contraction induced by electrical stimulation of the nerve than do NT2 and NT3. Homonuclear proton two-dimensional NMR methods were utilized to study the solution tertiary structure of NT2. A homology model-building method was employed to predict the structure of NT3. Comparison of the structures of these three toxins shows that the surface conformation of NT1 facilitates the substituted base residues, Arg28, Arg30, and Arg36, to occupy the favorable spatial location in the central region of loop 11, and the cation groups of all three arginines face out of the molecular surface of NT1 This may contribute greatly to the higher binding of NT1 with AchR compared to NT2 and NT3.
关键词	Short-chain Neurotoxin Homology Model Building Potential Surface Concave Face
资助者	This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation.
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收录类别	SCI
语种	英语
资助者	This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation. ; This work was supported by the Grant 39670165 from the Chinese Natural Science Foundation.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5245
专题	其他
通讯作者	jfw@sun5.ibp.ac.cn
作者单位	1.National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, PR China 2.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences Kunming, Yunnan 650223, PR China
推荐引用方式 GB/T 7714	Cheng Y,Meng QX,Wang WY,et al. Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3[J]. BIOCHIMICA ET BIOPHYSICA ACTA,2002,1594(2):353-363.
APA	Cheng Y,Meng QX,Wang WY,Wang JF*,&jfw@sun5.ibp.ac.cn.(2002).Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3.BIOCHIMICA ET BIOPHYSICA ACTA,1594(2),353-363.
MLA	Cheng Y,et al."Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3".BIOCHIMICA ET BIOPHYSICA ACTA 1594.2(2002):353-363.

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