Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms

	Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms
	Jin Y; Lu QM; Zhou XD; Zhu SW; Li R; Wang WY; Xiong YL*; wangwy@mail.kiz.ac.cn; jinyangkiz@hotmail.com
	2003
发表期刊	TOXICON
ISSN	0041-0101
卷号	42 期号:5 页码:539-547
合作性质	其它
摘要	Three 26 kDa proteins, named as TJ-CRVP, NA-CRVP1 and NA-CRVP2, were isolated from the venoms of Trimeresurus jerdonii and Naja atra, respectively. The N-terminal sequences of TJ-CRVP and NA-CRVPs were determined. These components were devoid of the enzymatic activities tested, such as phospholipase A(2), arginine esterase, proteolysis, L-amino acid oxidase, 5'nucleotidase, acetylcholinesterase. Furthermore, these three components did not have the following biological activities: coagulant and anticoagulant activities, lethal activity, myotoxicity, hemorrhagic activity, platelet aggregation and platelet aggregation-inhibiting activities. These proteins are named as cysteine-rich venom protein (CRVP) because their sequences showed high level of similarity with mammalian cysteine-rich secretory protein (CRISP) family. Recently, some CRISP-like proteins were also isolated from several different snake venoms, including Agkistrodon blomhoffi, Trimeresurus flavoviridis, Lanticauda semifascita and king cobra. We presumed that CRVP might be a common component in snake venoms. Of particular interest, phylogenetic analysis and sequence alignment showed that NA-CRVP1 and ophanin, both from elapid snakes, share higher similarity with CRVPs from Viperidae snakes.
关键词	Trimeresurus Jerdonii Naja Atra Cysteine-rich Venom Protein
资助者	This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M).
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收录类别	SCI
语种	英语
资助者	This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). ; This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5265
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	wangwy@mail.kiz.ac.cn; jinyangkiz@hotmail.com
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, People’s Republic of China
推荐引用方式 GB/T 7714	Jin Y,Lu QM,Zhou XD,et al. Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms[J]. TOXICON,2003,42(5):539-547.
APA	Jin Y.,Lu QM.,Zhou XD.,Zhu SW.,Li R.,...&jinyangkiz@hotmail.com.(2003).Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms.TOXICON,42(5),539-547.
MLA	Jin Y,et al."Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms".TOXICON 42.5(2003):539-547.

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