Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom

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	Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom
	Zhou XD 1,2,3; Jin Y 1; Lu QM 1; Li DS 1; Zhu SW 1; Wang WY 1; Xiong YL*1; toxinlab@mail.kiz.ac.cn
	2004
发表期刊	Comparative Biochemistry and Physiology Part B
ISSN	1096-4959
卷号	137 期号:2 页码:219-224
合作性质	其它
摘要	A chymotrypsin inhibitor, designated NA-CI, was isolated from the venom of the Chinese cobra Naja atra by three-step chromatography. It inhibited bovine alpha-chymotrypsin with a Ki of 25 nM. The molecular mass of NA-CI was determined to be 6403.8 Da by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) analysis. The complete amino acid sequence was determined after digestion of S-carboxymethylated inhibitor with Staphylococcus aureus V8 protease and porcine trypsin. NA-CI was a single polypeptide chain composed of 57 amino acid residues. The main contact site with the protease (P1) has a Phe, showing the specificity of the inhibitor. NA-CI shared great similarity with the chymotrypsin inhibitor from Naja naja venom (identities=89.5%) and other snake venom protease inhibitors.
关键词	Amino Acid Sequence Chinese Cobra Chymotrypsin Inhibitor Isolation Naja Atra Snake Venom
资助者	This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M.
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收录类别	SCI
语种	英语
资助者	This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. ; This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5317
专题	其他科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	toxinlab@mail.kiz.ac.cn
作者单位	1.Department of Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 650223 Kunming, Yunnan, PR China 2.Graduate School of the Chinese Academy of Sciences, 100039 Beijing, PR China 3.Faculty of Biological and Chemical Engineering, Kunming University of Science and Technology, 650224 Kunming, Yunnan
推荐引用方式 GB/T 7714	Zhou XD,Jin Y,Lu QM,et al. Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom[J]. Comparative Biochemistry and Physiology Part B,2004,137(2):219-224.
APA	Zhou XD.,Jin Y.,Lu QM.,Li DS.,Zhu SW.,...&toxinlab@mail.kiz.ac.cn.(2004).Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom.Comparative Biochemistry and Physiology Part B,137(2),219-224.
MLA	Zhou XD,et al."Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom".Comparative Biochemistry and Physiology Part B 137.2(2004):219-224.

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