Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom

	Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom
	Jin Y; Lu QM; Chen RQ; Wu JB; Xiong YL*; jinyang@mail.kiz.ac.cn
	2005
发表期刊	TOXICON
ISSN	0041-0101
卷号	45 期号:3 页码:353-360
合作性质	其它
摘要	A fibrinogen-clotting enzyme designed as jerdonobin-II was isolated from the venom of Trimeresurus jerdonii. It differed in molecular weight and N-terminal sequence with the previously isolated jerdonobin, a thrombin-like enzyme from the same venom. The enzyme consists of a single polypeptide chain with molecular weights of 30,000 and 32,000 under non-reducing and reducing conditions, respectively. Jerdonobin-II showed weak fibrinogen clotting activity and its activity unit on fibrinogen was calculated to be less than one unit using human thrombin as standard. The precursor protein sequence of jerodonobin-II was deduced from cloned cDNA sequence. The sequence shows high similarity (identity=89%) to TSV-PA, a specific plasminogen activator from venom of T. stejnegeri. Despite of the sequence similarity, jerdonobin-II was found devoid of plasminogen activating effect. Sequence alignment analysis suggested that the replacement of Lys239 in TSV-PA to Gln239 in jerdonobin-II might play an important role on their plasminogen activating activity difference.
关键词	Snake Venom Thrombin Plasminogen Fibrinogen Trimeresurus Jerdonii
资助者	This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission.
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收录类别	SCI
语种	英语
资助者	This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. ; This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5349
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	jinyang@mail.kiz.ac.cn
作者单位	Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, East Jiao-Chang Road, Kunming 650223, Yunnan, People’s Republic of China
推荐引用方式 GB/T 7714	Jin Y,Lu QM,Chen RQ,et al. Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom[J]. TOXICON,2005,45(3):353-360.
APA	Jin Y,Lu QM,Chen RQ,Wu JB,Xiong YL*,&jinyang@mail.kiz.ac.cn.(2005).Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom.TOXICON,45(3),353-360.
MLA	Jin Y,et al."Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom".TOXICON 45.3(2005):353-360.

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