Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom

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	Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom
	Wei JF 1,2; Yang HW 1; Wei XL 2; Qiao LY 2; Wang WY 3; He SH*1,2; weijifu@hotmail.com ; shoahenghe@ hotmail.com
	2009
发表期刊	TOXICON
ISSN	0041-0101
卷号	54 期号:3 页码:262-271
合作性质	其它
摘要	L-Amino acid oxidases (LAAOs) are widely distributed in snake venoms, which contribute to the toxicity of venoms. However, LAAO from Bungarus fasciatus (B. fasciatus) snake venom has not been isolated previously. In the present study, LAAO from B. fasciatus snake venom was purified by SP-Sepharose HP anion exchange chromatography followed by Heparin-Sepharose FF affinity chromatography procedure and the purified enzyme was named BF-LAAO. BF-LAAO presented an estimated molecular weight of 55 kDa in SDS-PAGE and an apparent molecular weight of 70 kDa in size-exclusion chromatography suggesting that BF-LAAO is a monomeric protein. Kinetics studies showed that BF-LAAO was very active against L-Tyr, L-Asp, L-Phe, L-Glu, L-Trp, L-His, L-Gln, L-Ile, L-Met, L-Leu and moderately active against L-Lys, L-Arg, L-Ala and L-Asn. BF-LAAO exhibited a cytotoxic effect on A549 cells and caused up to 41.2% apoptosis of A549 cells following 12 h incubation period. In the mouse peritoneum, BF-LAAO provoked a marked increase in the number of neutrophils, lymphocytes and macrophages following injection. It also induced rabbit platelet aggregation in a dose-dependent manner. At 3 h following injection, BF-LAAO elicited severe inflammation in the gastrocnemius muscles of mice, but failed to induce significant organ damage. In conclusion, the cytotoxic and proinflammatory activities of BF-LAAO could be the main cause of the local inflammation, which helps us to understand the pathogenesis of snakebite.
关键词	L-amino Acid Oxidases Bungarus Fasciatus Myonecrosis Apoptosis Inflammation
资助者	This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032).
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收录类别	SCI
语种	英语
资助者	This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032). ; This project was sponsored by the Major State Basic Research Program of China (973 Program) (No. 2007CB512400) and the National Natural Science Foundation of China (No. 30801016, 30570813, 30772032).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5391
专题	其他
通讯作者	weijifu@hotmail.com; shoahenghe@ hotmail.com
作者单位	1.Clinical Research Centre, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China 2.Allergy and Inflammation Research Institute, The Shantou University Medical College, Shantou, Guangdong 515031, China 3.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Wei JF,Yang HW,Wei XL,et al. Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom[J]. TOXICON,2009,54(3):262-271.
APA	Wei JF.,Yang HW.,Wei XL.,Qiao LY.,Wang WY.,...&shoahenghe@ hotmail.com.(2009).Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom.TOXICON,54(3),262-271.
MLA	Wei JF,et al."Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom".TOXICON 54.3(2009):262-271.

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