| Development of zona-free hamster ova to blastocysts in vitro | |
| Ji W*1,3; Bavister BD2,3,4; wji@mail.kiz.ac.cn | |
| 2000 | |
| 发表期刊 | THERIOGENOLOGY
 |
| ISSN | 0093-691X |
| 卷号 | 54期号:6页码:827-834 |
| 合作性质 | 其它 |
| 摘要 | The zona pellucida (ZP) enclosing the mammalian ovum is important for its protection and for initial stages of fertilization, but the role of the ZP during embryo development is less clear. This study was designed to investigate if the hamster ZP is needed for embryo development from 1-cell to blastocyst in vitro, and to compare methods for removing the ZP. A total of 395 hamster pronucleate ova were collected 10 h post activation from superovulated, mated female hamsters. The ZP was removed from some ova using either 0.05% pronase, 0.05% trypsin or acid Tyrode's solution. To prevent ZP-free ova from sticking together, they were cultured singly in 30-50 microL drops of HECM-6 culture medium together with ZP-intact ova as controls. There was no significant difference among treatment groups in embryo development to blastocyst: 36/87 (42%) in the ZP intact group; 35/75 (47%) in the pronase-treated ZP-free group; 37/74 (50%) in the trypsin-treated ZP-free group; and 37/71 (52%) in the acid-treated ZP-free group. These results indicate that 1) the ZP is unnecessary for hamster embryo development in vitro from the pronucleate ovum stage to blastocyst; 2) none of the three ZP-removal methods was detrimental to embryo development; 3) embryos do not need to be cultured in groups during in vitro development from 1-cell to blastocyst. |
| 关键词 | Zona Pellucida Zona Removal Embryo Development Blastocyst |
| 资助者 | Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. ; Supported by the John D. and Catherine T. MacArthur Foundation; the NICHD (National Program for Non-Human in vitro Fertilization and Embryo Development, grant no. HD22023); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 960364. We are grateful to Dr. John Eppig for his constructive comments on the manuscript. |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5539 |
| 专题 | 生殖与发育生物学 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 作者单位 | 1.Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, China 2.Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin USA 3.China-US. Primate Biology Laboratory, Kunming, China and Madison, USA. 4.Present address: Dept. of Biological Sciences, University of New Orleans, and Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA. |
| 推荐引用方式 GB/T 7714 | Ji W*,Bavister BD,wji@mail.kiz.ac.cn. Development of zona-free hamster ova to blastocysts in vitro[J]. THERIOGENOLOGY,2000,54(6):827-834. |
| APA | Ji W*,Bavister BD,&wji@mail.kiz.ac.cn.(2000).Development of zona-free hamster ova to blastocysts in vitro.THERIOGENOLOGY,54(6),827-834. |
| MLA | Ji W*,et al."Development of zona-free hamster ova to blastocysts in vitro".THERIOGENOLOGY 54.6(2000):827-834. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 200054827.pdf（1046KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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